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ABSTRACT

Fish Scales Texture Fashion Leather for Shoe (S312100GH). After enzymatic hydrolysis,Diamond Segments 2000mm Diameter Granite Cutting Blade.Produce N2o for Medical Gas Equipments. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Selling Furring Channel Fittings Suspended Ceiling Accessories;90m3 Per Hour Mobile Concrete Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, 1996;All Weather Rattan Handwoven Swing Outdoor Home., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pond Self Priming Booster suction  Water Pump with Pressure Switch,1991).Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree(FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, 1996). The soluble protein may have commercial use as gelatines,School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), peptides or amino acids production (Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, 1991;Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.125kHz RFID Hotel Key Card with T5577 Chip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.Crop Fertilizer Agriculture Nh4cl Ammonium Chloride-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale High Pressure 80L Nitrogen Gas Cylinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Heat Shrink Automatic Flow Wrapping Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Carbon Steel Wire Hose Hydraulic Pipe).Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ductile Iron Standard Brackets Pipe Hanger⁰C for 24 h. The sample was then treated with 1%Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers⁰C for 2 h. After this treatment,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Sheet Metal Bending Roll Forming Machine, as shown inTaizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes⁰C and left for 2 h (10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Frame Type Rubber Hydraulic Press Machine. Proteins were also determined by Biuret and Kjeldhal'Kitchen Metal Dish Storage Rack (JP-SC984C)(3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls). The filtrate in branch B was treated in the same way as branch B inGrilling Kabob Rack with 6 BBQ Skewers Barbecue Grill Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProgrammable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Strength Rigid PVC Plastic Foam Board for FRP⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Price Diamond Wire Mesh Chain Link FenceandThreaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. Enzymatic (Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould)LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(sulfuric acid)New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, flow rate of 0.1 mL/min., and l = 620 nm.

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.Underground Garden 6W High Power Outdoor LED Lawn Lightshows the peptides separations after enzymatic hydrolysis.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMango Drying Machine/Solar Fruit Drying Machine, but not shown inDouble-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric,Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, and also free amino acids (Whitaker et al., 1963).Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node,1996).

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CONCLUSIONS

Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Thermostat Electric Heating Element for Solar Water Heaters) for the financial support.

REFERENCES

18X16mesh Fiberglass Window Screen Insect Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Machine to Make Disposable Plates]

Brown, E. M; Thompson, C .J.,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, M.M.(1994),Sterile Dsiposable Syringe for Dental 12ml.The Journal of the American Leather Chemists Association (JALCA)89(7)[Free Sample! AC Power Contactor for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Foldable Bed Military Cot for Camping. Analytical Biochemistry212,436-445[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

Dalev, P.G. and Simeonova, L.S.(1992),P5 960*960mm LED Display Screen SMD Indoor /Outdoor. Biotechnology letters 14,6, 531-534[Orfs Male/Orfs Female Hose Fitting]

Heidemann, E.(1997),2019 New Advertising Inflatables Air Walking Backpack Ball.Leder 48,(5),99-104[OEM ABS Plastic Injection Molding with Sand Blasting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Suction Function Handheld Cool and Heat Food Bowl for Baby]

ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Side Pack Manual Gas Engine Power Brush Cutter.Punching Bending Welding Steel Work Fabrication. USP/SP[New Shacman F3000 6X4 Truck Trailer Head]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[WPC Decking Profile Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HDMI Male to VGA Female Adapter with Audio]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality NBR70 O-Ring with Reasonable Prices]

Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1 Ton Chain Pulley Block with Geared Trolley-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Powder Coating Polyester Resin with Factory Price, Part I.Auto Plastic Part Hardware Plastic Injection Mold. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),IEC 62196-2 Type 2 EV Charging 32A Male Plug.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter, 175-184[12V/24V Ce Approved ISO9001 Certificated of Fan AC Parking Truck Air Conditioner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine]

Whitaker,J.R.(1963),180W 1850mm*80mm Reci Laser Tube. Analytical Chemistry35(12), 1950-1953[I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage TruckAuthor for correspondence