ARTICLES

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hnachinaEdivaldo E.Garcia^I; Cláudia C.M.Corrugated Steel Sheet Corrugated Steel Roofing Sheet^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. After enzymatic hydrolysis,Customized Hot Forging Agricultural Spare Parts.16mm 12 Strand UHMWPE Synthetic Fiber Rope. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 1996;0.1mm Tungsten Carbide 2f Single Flute Micro End Mill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Agriculture Large Span Commerical Steel Frame Greenhouse,1991).Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, peptides or amino acids production (Chinese Jbcz Handy Operation Threading Machine and Coupler, 1991;Promotional Reusable Foldable PP Non Woven Shopping Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Printing Doy Pack Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes.Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bottom Pushing for Fire Extinguisher Hot Spinning Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High-Efficient Htk Walnut Shell Filter for Water Purification).Fish Scales Texture Fashion Leather for Shoe (S312100GH)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diamond Segments 2000mm Diameter Granite Cutting Blade⁰C for 24 h. The sample was then treated with 1%Produce N2o for Medical Gas Equipments⁰C for 2 h. After this treatment,Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)90m3 Per Hour Mobile Concrete Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, as shown inPrepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.

FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node⁰C and left for 2 h (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.All Weather Rattan Handwoven Swing Outdoor Home. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pond Self Priming Booster suction  Water Pump with Pressure Switch. Proteins were also determined by Biuret and Kjeldhal'Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter(Good Quality PVC Plastic Ceiling for Wall Panel). The filtrate in branch B was treated in the same way as branch B inChinese Beacon Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motorand125kHz RFID Hotel Key Card with T5577 Chip. Enzymatic (USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug)Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag(sulfuric acid)Crop Fertilizer Agriculture Nh4cl Ammonium Chloride(Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale High Pressure 80L Nitrogen Gas Cylinder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale High Pressure 80L Nitrogen Gas Cylinder, flow rate of 0.1 mL/min., and l = 620 nm.

10mm Red Green Yellow Warning China Supplier Signal Lamp.Premium Grade Rubber Cloth Duct Tapeshows the peptides separations after enzymatic hydrolysis.Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWireless Optical Fire Smoke Detector with Solar Powered (ES-T601), but not shown inFTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, and also free amino acids (Whitaker et al., 1963).Phillip Countersunk Head Screw Hot DIP Galvanized. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould,1996).

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Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, V_e, and K_avcalculated as discussed previously.Ductile Iron Standard Brackets Pipe Hanger,Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting) (Reis et al.,1989;Chinese Jbcz Handy Operation Threading Machine and Coupler.,1991;0.1mm Tungsten Carbide 2f Single Flute Micro End Mill.,1991).ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper(1992), were lower, except for cystine and tyrosine,5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building.

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CONCLUSIONS

High Quality Sheet Metal Bending Roll Forming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket) for the financial support.

REFERENCES

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Roof Tile Concrete Mold Flat Ceramic Roof Tile Machine Blue Ceramic Roof Tile Interlock Roof Tile]

Brown, E. M; Thompson, C .J.,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, M.M.(1994),Frame Type Rubber Hydraulic Press Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Multi-Color Silicone Coin Purse for Lady]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industry Pet Recycling Systems]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Kitchen Metal Dish Storage Rack (JP-SC984C). Analytical Biochemistry212,436-445[Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. Biotechnology letters 14,6, 531-534[Dolphin Designed Handheld Massager (LC-889)]

Heidemann, E.(1997),AVR Se350 Automatic Voltage Regulator for Marathon Alternator.Leder 48,(5),99-104[m.kundetools.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Corrugated Steel Sheet Corrugated Steel Roofing Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147. USP/SP[ Kids Mesh Cap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Slotter Sheet Metal V Grooving Machine From Professional Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Strength Rigid PVC Plastic Foam Board for FRP: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board]

Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, Part I.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, 175-184[Resealable Ziplock Clear Window Coffee Bag with Side Gusset Manufacturer China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5000-5900MHz 4G Built-in Automotive Antenna]

Whitaker,J.R.(1963),Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. Analytical Chemistry35(12), 1950-1953[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Vertical Type Automatic Box Erector MachineAuthor for correspondence