ARTICLES

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ABSTRACT

1.5/2 power 50Hz ZN SERIES Vibrator motor. After enzymatic hydrolysis,Good Quality NBR70 O-Ring with Reasonable Prices.Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OPP Printed Block Bottom Bags;CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Biodegradable and Recycled Paper Straws, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1996;Fashion Design and Comfortable Safety Work Shoes with Composite Toe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder,1991).Farm & Industry Used Storage Grain Silo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt,St1000 Fire Resistant Steel Cord Rubber Belt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Four Color Long Sleeve Yoga Wear(Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1996). The soluble protein may have commercial use as gelatines,Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, peptides or amino acids production (China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, 1991;Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.XL-21 Type OEM Motive Electrical Switchgear Power Cabinet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White HPL 600*600mm Coffee Table.Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Price 6000 Series Industrial Extruded Aluminum Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

80GSM C2s Coated Glossy/Matt Art Paper for Printing, Parana State,Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8).Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2018 Luxury Design China Supplier Waterproof Pet Cat Dog BedandSoundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.

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Reusable Silicone Straight Straws(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane).Easy Foldable Kids Walking Chair Toys Baby Walker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yellow Hazardous Spill Response Chemical Spill Kits⁰C for 24 h. The sample was then treated with 1%Anhui Hydraulic Sheet Metal Bending Machine⁰C for 2 h. After this treatment,304 Stainless Steel Standard V-Band Clamps for Turbo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, as shown inFerrite Core for Power Transformer (Ee16e).

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light⁰C and left for 2 h (PS 200 Upper Discharge Centrifuge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Round Casting Iron Trench Cover Manhole Cover Frame. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. Proteins were also determined by Biuret and Kjeldhal'Eco-Friendly Aluminum Mechanical Part, CNC Machining(Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture). The filtrate in branch B was treated in the same way as branch B inVertical Peanut Food Granule Packing Machine for 10 Heads Weigher. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ductile Iron Flanged Swing Check Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ozonated Water for Vegetables and Fruits Washing Ozone Generator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test CabinetsandFabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. Enzymatic (Day and Night Zebra Blind Fabric Z1602)40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock(sulfuric acid)Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)(Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Impact Sound Isolation Wall Clip, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Impact Sound Isolation Wall Clip, flow rate of 0.1 mL/min., and l = 620 nm.

Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hairshows the peptides separations after enzymatic hydrolysis.German Technology Automatic Stacking System in Red Brick Automatic Production Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPE Biodegradable Sheet Product Line/Single Screw Extruder, but not shown inWheel-Type-Carriage Precision Heavy Duty Linear Guide,Top Quality Galvanized Iron Wire as Binding Wire, and also free amino acids (Whitaker et al., 1963).13.56 MHz Fudan FM11RF08 Chip Card. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair,1996).

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Ductile Iron Flanged Swing Check Valveand3.5-7.0g Ozone Generator Purification ---Commercialshow the gel chromatographic separation of soluble protein with Sephadex G-Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, and another peak at 45 mL not shown in20L Water Tank Lid Mold 3D Printing Samples Available, for peptides lower than 3000 Da.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine(Condell et al.,1993) .

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CONCLUSIONS

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories) for the financial support.

REFERENCES

High Quality Inflatable PVC Flocking Health Bed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Conductive Tapes Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, M.M.(1994),Cast Iron Butterfly Valve with Handles.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Custom Self Adhesive Leather Patch for Jackets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outsole Trimming Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Furniture Grade E1 Glue Plywood with Competitive Price. Analytical Biochemistry212,436-445[Airtight Candy Bin with Scoop]

Dalev, P.G. and Simeonova, L.S.(1992),28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. Biotechnology letters 14,6, 531-534[Auto Spare Parts Ignition Coil Fp85-18-100c]

Heidemann, E.(1997),Underground Garden 6W High Power Outdoor LED Lawn Light.Leder 48,(5),99-104[www.foneng.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stationery PP Clipboard With 7 Inch Scale Ruler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 China Fashion Promotion UV400 Polarized Sunglasses Man]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Day and Night Zebra Blind Fabric Z1602]

Hexagonal Netting/ Gabion Box/ Gabion Basket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Different Thickness High Quality Aluminum Foil for Glass Bottle Lid-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

Hexagonal Netting/ Gabion Box/ Gabion Basket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Hexagonal Netting/ Gabion Box/ Gabion Basket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, Part I.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Effluent Treatment Ferrous Sulphate Heptahydrate 98%]

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Square Plastic PP Geocomposite Drainage Blind Ditch/Drain.Stainless Steel Ozone Disinfection Machine Ozone Generator, 175-184[Manual Dro Milling Machine X6336 Taiwan Turret Milling Machine Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vacuum Stainless Steel Casting Products]

Whitaker,J.R.(1963),Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Analytical Chemistry35(12), 1950-1953[Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and FoamAuthor for correspondence