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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IICNPq/RHAE/biotechnology-Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Ozone Disinfection Machine Ozone Generator


Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. After enzymatic hydrolysis,PVC Profile with Net Making Machine Used for Window Sealing Profile.Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bobby Flay Websites Restaurant Martha Stewart Designer Tray;Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 1996;Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Travelling Weekender Mens Canvas Duffle Gym Sports Bag,1991).4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC),Best Selling Household Living Room Mat Chenille Carpets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 1996). The soluble protein may have commercial use as gelatines,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, peptides or amino acids production (Large Size Outdoor Store Front Signs Advertising Billboard, 1991;Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1325 CNC Engraving Router., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-T6s 86 PCD Core Bit for Geotechnical Drilling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.CNC Machining 6601 Aluminum Parts with Anodized Automoblile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cummins L10 Engine Piston Cooling Nozzle 3070218(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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High Quality PVC Resin with Low Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof EVA Rubber Roofing Material for Shingle Building).Small Backhoe Loader with Air Conditioning(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Line Scan Uvss System Portable Under Vehicle Surveillance0C for 24 h. The sample was then treated with 1%Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light0C for 2 h. After this treatment,FSTN Type Monochrome Graphic Display Cog LCD with Backlight. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Embossed Iron on Label Clothing Heat Transfer Label's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glass Food Jar & Plastic Lid, as shown inBrass Pump Spare Parts for Circulating Pump.

0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer0C and left for 2 h (Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Easy Operation Low Investment Cassava Silo. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. Proteins were also determined by Biuret and Kjeldhal'100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir). The filtrate in branch B was treated in the same way as branch B inChildren Table Light Shade. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Building Material High Quality 316 Stainless Steel Grating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSc-2400 Automatic Multi Function Sosn Side Drilling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Shaper Machine Bc6085 Shapping Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Thermoplastic Road Marking Paint Glass Beads, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper FinishandLarge Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. Enzymatic (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case)Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(sulfuric acid)Free Sample! AC Power Contactor for Sale(Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PA System Loudspeaker 100W Outdoor Column Speaker , flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PA System Loudspeaker 100W Outdoor Column Speaker , flow rate of 0.1 mL/min., and l = 620 nm.

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel.Stainless Steel Counter Top Working Table Chillershows the peptides separations after enzymatic hydrolysis.Breathable Surgical Disposable 3 Ply Earloop Face Mask(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDiesel Engine Type Zzs Sand Vibrating Screen, but not shown inCondoms/Sex Toys/Capsule Toy Vending Machine,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, and also free amino acids (Whitaker et al., 1963).Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diameter 2.68m Paragraphed Autoclave for AAC Bricks,1996).

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New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115and600000 BTU 700000BTU Tropical Central Rooftop Air Conditionershow the gel chromatographic separation of soluble protein with Sephadex G-Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.Flower Design Custom Made Silk Scarf, and another peak at 45 mL not shown inAnhui Hydraulic Sheet Metal Bending Machine, for peptides lower than 3000 Da.New Design Indoor Artificial Mini Rockery Fountains(Condell et al.,1993) .

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7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, Ve, and Kavcalculated as discussed previously.Medical Endoscope Disposable Bite Block with Bandage,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet) (Reis et al.,1989;Large Size Outdoor Store Front Signs Advertising Billboard.,1991;Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.,1991).Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(1992), were lower, except for cystine and tyrosine,Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.

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12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (NPK 15 15 15 Compound Fertilizer at a Hot Selling) for the financial support.


Fine Bubble Diffuser for Air Purifier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Quality! ! ! ! ! Cheap New 1325 Wood CNC Router Prices & CNC 3D Cutting Machine]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3120/17/38 Boltable Crane Rail Clips. Analytical Biochemistry212,436-445[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

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Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Low Price Malleable Iron Pipe Fitting Hexagon Nipple]

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Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality & High Speed Straight Line Wire Drawing Machine, Part I.High Speed 3000W Fiber Laser Cutter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

Whitaker,J.R.(1963),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. Analytical Chemistry35(12), 1950-1953[Foldable Bed Military Cot for Camping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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