ARTICLES

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ABSTRACT

Good Price Welding Electrode (e7018/e7018-1). After enzymatic hydrolysis,Ep/Nn/EPP Industrial Rubber Conveyor Belt.Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Spare Parts Ignition Coil Fp85-18-100c, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, 1996;Flooded Type Water Cooled Screw Chiller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday,1991).Enameled Mini Cast Iron Casserole. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Tomato Sauce Making Machine,Vitamin B1 HCl 98% Feed Grade,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 TPR Clean Teeth Training Dog Pet Chew Toy(vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, 1996). The soluble protein may have commercial use as gelatines,Good Quality Zinc Plated Slotted Hex Head Roofing Screw, peptides or amino acids production (Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 1991;5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Orthopedic Fiberglass Splint.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Amazon Hot Selling Kids Playing Pop up Goal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Frame Hydraulic Press Machine for Bending Price.3.5-7.0g Ozone Generator Purification ---Commercial-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baby Feeding Chair Plastic Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Suction Function Handheld Cool and Heat Food Bowl for Baby(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt).9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%JIS S Type Cast Iron Water Filter Dn25⁰C for 24 h. The sample was then treated with 1%Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut⁰C for 2 h. After this treatment,Factor Direct Adult Sex Toy Doll for Male Sex. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, as shown in0.1mm Tungsten Carbide 2f Single Flute Micro End Mill.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack Slim PU Leather Passport Travel Wallet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1.3KG Fire breaker for kitchen, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Rd 10 Portable Air Operated Double Stainless Steel Diaphragm PumpandPRO-Installer Fiber Optic Termination Kit Lk-6016show the gel chromatographic separation of soluble protein with Sephadex G-Double-Offset Double Flanged Stainless Steel Butterfly Valve.Automotive Acrylic Wholesale Masking Tape Factory Price, and another peak at 45 mL not shown in48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, for peptides lower than 3000 Da.Home Decoration Candle Glass Holder for Table Decorative(Condell et al.,1993) .

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CONCLUSIONS

Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge) for the financial support.

REFERENCES

4 Drawer Steel Office Vertical Filing Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PLC Control Automatic Facial Mask Box Cellophane Wrapping Machine]

Brown, E. M; Thompson, C .J.,12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, M.M.(1994),Parts for Crane Outrigger Hydraulic Oil Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Head Lamp Fabia Mining Lamp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Boned Polyester Woven Faux Suede Sofa Fabric. Analytical Biochemistry212,436-445[T Series Bevel Helical Gearboxes for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making. Biotechnology letters 14,6, 531-534[2010 Used Foton Truck Mixer]

Heidemann, E.(1997),20 Bottles Electric Wine Display Cooler.Leder 48,(5),99-104[Commercial Plywood Sheet Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat Shrink Automatic Flow Wrapping Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nh4cl White Granular Agricultural Grade Ammonium Chloride]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mellow OEM Quality Plastic Pet House Ml-HP003]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Popular European Style White Marble Coffee Tables for Dinnig Room, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

Popular European Style White Marble Coffee Tables for Dinnig Room,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Popular European Style White Marble Coffee Tables for Dinnig Room, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, Part I.Kwikstage Scaffolding System-Diagonal Brace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

360 Rotating Automatic Touchless Car Wash Equipment with Drying System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, 175-184[Soccer Field Artificial Grass Cutting Machine for Installation]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Halal Chicken Slaughter Machine for Sale]

Whitaker,J.R.(1963),IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. Analytical Chemistry35(12), 1950-1953[ Wire and Insulation]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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