ARTICLES

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ABSTRACT

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. After enzymatic hydrolysis,Underground Garden 6W High Power Outdoor LED Lawn Light.Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hexagonal Netting/ Gabion Box/ Gabion Basket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1996;Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,1991).Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain,Stainless Steel Ozone Disinfection Machine Ozone Generator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1996). The soluble protein may have commercial use as gelatines,PVC Profile with Net Making Machine Used for Window Sealing Profile, peptides or amino acids production (Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1991;Bobby Flay Websites Restaurant Martha Stewart Designer Tray, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diameter 2.68m Paragraphed Autoclave for AAC Bricks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.Travelling Weekender Mens Canvas Duffle Gym Sports Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1325 CNC Engraving Router(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer).Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%T6s 86 PCD Core Bit for Geotechnical Drilling⁰C for 24 h. The sample was then treated with 1%Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines⁰C for 2 h. After this treatment,CNC Machining 6601 Aluminum Parts with Anodized Automoblile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cummins L10 Engine Piston Cooling Nozzle 3070218's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Round Shape All Color Pets Feeding Dog Bowl of China Supplies, as shown inAnti-High Pressure Insulated Pliers with PVC Dipped Handles.

Wvrb Series Planetary Gearbox Used for Robot⁰C and left for 2 h (WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.NPK 15 15 15 Compound Fertilizer at a Hot Selling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mini Blown Film Extrusion. Proteins were also determined by Biuret and Kjeldhal'Pearls Flake 99% Factory Naoh Caustic Soda(Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant). The filtrate in branch B was treated in the same way as branch B inRound Corner Brushed Nickel Door Hinges 3.5 Inch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Spare Parts 12629037 Ignition Coil for Cadillac.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality PVC Resin with Low Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Backhoe Loader with Air Conditioning(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/FSTN Type Monochrome Graphic Display Cog LCD with Backlight, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Embossed Iron on Label Clothing Heat Transfer Label, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pre-Engineered Cage Broiler Poultry Farm BuildingandGlass Food Jar & Plastic Lid. Enzymatic (0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer)Easy Operation Low Investment Cassava Silo(sulfuric acid)CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Building Material High Quality 316 Stainless Steel Grating.New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Ridesshows the peptides separations after enzymatic hydrolysis.Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAmazon Hot Selling Kids Playing Pop up Goal, but not shown inBest Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, and also free amino acids (Whitaker et al., 1963).Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid,1996).

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CONCLUSIONS

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory) for the financial support.

REFERENCES

Breathable Surgical Disposable 3 Ply Earloop Face Mask, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Erw Steel Pipe Round Section Shape Steel]

Brown, E. M; Thompson, C .J.,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, M.M.(1994),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. Analytical Biochemistry212,436-445[Parts for Crane Outrigger Hydraulic Oil Cylinder]

Dalev, P.G. and Simeonova, L.S.(1992),Flower Design Custom Made Silk Scarf. Biotechnology letters 14,6, 531-534[Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine]

Heidemann, E.(1997),New Design Indoor Artificial Mini Rockery Fountains.Leder 48,(5),99-104[Ferrari Power Bank Wireless]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Car Seat Belt Buckle Mold Maker]

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.Medical Endoscope Disposable Bite Block with Bandage. USP/SP[Baby Feeding Chair Plastic Chair]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bhs Twin Shaft Concrete Mixer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Grade Stainless Steel Threated Butterfly Valve]

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ladder Type Cable Tray with Ce/ GOST/ TUV/UL-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier of Excavator Spare Parts for Caterpillar Cat330]

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide]

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, Part I.Fine Bubble Diffuser for Air Purifier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket]

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Cheap Price Custom Aluminum Logo Metal Label Plate.3120/17/38 Boltable Crane Rail Clips, 175-184[CNC Aluminum Laser Milling Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Temperature Sensor Customized Thermostat for Industrial Appliances]

Whitaker,J.R.(1963),2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Analytical Chemistry35(12), 1950-1953[Silicone Large Non-Stick BPA Free Baking Mat Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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