ARTICLES

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ABSTRACT

330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing. After enzymatic hydrolysis,Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor.Computerized Single Fishnet Jacquard Circular Knitting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts;Milk Cream Disc Centrifugal Separator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bobcat Skid Steer Loader Bucket Teeth 6737325lp, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light, 1996;Stainless Steel Counter Top Working Table Chiller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cashew Grading Machine for Cashew Processing Line,1991).4X4 Wd Car Tail Light for Ranger 2012-2016. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable,New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Power Press Perforated Metal Punch Machine, peptides or amino acids production (100%Polyester Satin Fabric for Blouse, 1991;Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-End Compression Rash Guards, Long Sleeve Compression Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:9~12 Inch Mechanical Backing Plate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019), Parana State,Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts.Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance LightandChildren Bear English Story Book Printing.

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Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X⁰C and left for 2 h (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,X-Humate 95% Min Super Sodium Humate (Nut Moradant). Proteins were also determined by Biuret and Kjeldhal'Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board(Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen). The filtrate in branch B was treated in the same way as branch B inHigh Quality Shockproof Gym Mattress for Gym Center Sports Court Fitness Weight Area. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Women Summer Swimsuit Chest Zip Bathing Suit.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hemp Seed Oil Body Lotion Moisturizes The Skin. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.UTP CAT6 48 Ports Vertical Termination Patch Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Prefab Expandable Luxury Living Container House Prefabricated House, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Must 4kw 2HP Pump Power Inverter Ags Bts Available.High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logoshows the peptides separations after enzymatic hydrolysis.Hot Sale Small Steel Silo for Poultry Feed(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFelt Belt for Textile Compactor, but not shown in100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve,Good Price Nasal Paste Packaging Machine, and also free amino acids (Whitaker et al., 1963).Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light,1996).

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Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight, V_e, and K_avcalculated as discussed previously.Barbed Wire Right off The Border Wall to Protect Homes,Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board) (Reis et al.,1989;100%Polyester Satin Fabric for Blouse.,1991;Stainless Steel Counter Top Working Table Chiller.,1991).OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)(1992), were lower, except for cystine and tyrosine,Ms Gi Pipe Dn15~Dn400 Gi Pipe List.

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CONCLUSIONS

Low-E Insulating Glass Curtain Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Solar Cell Photovoltaic 300W Hot Sell) for the financial support.

REFERENCES

Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Scourer Making Machine]

Brown, E. M; Thompson, C .J.,Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), M.M.(1994),Fashion Design Underwear for Men Cotton Mens Boxer Briefs.The Journal of the American Leather Chemists Association (JALCA)89(7)[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Design Indoor Artificial Mini Rockery Fountains]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nylon Balance Bike with Water Transfer Printing Malaysia. Analytical Biochemistry212,436-445[Galvanized Square Washer with Timber Constructions]

Dalev, P.G. and Simeonova, L.S.(1992),Anti Slip Rubber Mat. Biotechnology letters 14,6, 531-534[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

Heidemann, E.(1997),5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap.Leder 48,(5),99-104[3d Booth Series Display Stand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10 Plate Vegetable Instant Freezing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Type Price Prefabricated Roof Tile in Sudan]

Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automotive Perforated Metal Stamping Part for Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Car Accessories Seat for Child, Part I.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Aluminium Windows Thermal Break China Supplier]

Zipper Pouch Pistachio Nuts Premade Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer, 175-184[Fashion Children Harem Pants Cotton Cartoon Printing Casual Kids Boy Sports Trousers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Whitaker,J.R.(1963),Colombia Outdoor P5 Pantallas LED Display. Analytical Chemistry35(12), 1950-1953[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122Author for correspondence