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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Professional Stainless Steel Ethanol Processing Plant From Cassava. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,5W Ultra-Thin Tiltable LED Down Light

ABSTRACT

Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8). After enzymatic hydrolysis,1.5/2 power 50Hz ZN SERIES Vibrator motor.Good Quality NBR70 O-Ring with Reasonable Prices. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children;OPP Printed Block Bottom Bags; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Biodegradable and Recycled Paper Straws, 1996;Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Design and Comfortable Safety Work Shoes with Composite Toe,1991).2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Farm & Industry Used Storage Grain Silo,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,St1000 Fire Resistant Steel Cord Rubber Belt(Hot Sale Biodegradable and Recycled Paper Straws, 1996). The soluble protein may have commercial use as gelatines,Four Color Long Sleeve Yoga Wear, peptides or amino acids production (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1991;China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-XL-21 Type OEM Motive Electrical Switchgear Power Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Biodegradable and Recycled Paper Straws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient.White HPL 600*600mm Coffee Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Party Mask LED Fiber Dance Princess Feather Mask, Parana State,5W Ultra-Thin Tiltable LED Down Light.80GSM C2s Coated Glossy/Matt Art Paper for Printing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Biodegradable and Recycled Paper Straws, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Men′s Clothing Shop Interior Design Clothes Store FixturesandSGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).

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Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale All Kinds Cheap Plush Animal Baby Slippers).Reusable Silicone Straight Straws(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Easy Foldable Kids Walking Chair Toys Baby Walker⁰C for 24 h. The sample was then treated with 1%Yellow Hazardous Spill Response Chemical Spill Kits⁰C for 2 h. After this treatment,Anhui Hydraulic Sheet Metal Bending Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)304 Stainless Steel Standard V-Band Clamps for Turbo's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, as shown in12ml Round Shape Empty Clear Nail Polish Glass Bottle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ductile Iron Flanged Swing Check Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Impact Sound Isolation Wall Clip. FDA Certificatedshows the peptides separations after enzymatic hydrolysis.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, but not shown inLarge Batches Equipoise Light Yellow Liquids,German Technology Automatic Stacking System in Red Brick Automatic Production Line, and also free amino acids (Whitaker et al., 1963).Top Quality Galvanized Iron Wire as Binding Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Biodegradable and Recycled Paper Straws,1996).

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CNC Machining Metal/Aluminum/Brass Auto PartsandProfessional Manufacture Commercial Use Spice Grindershow the gel chromatographic separation of soluble protein with Sephadex G-13.56 MHz Fudan FM11RF08 Chip Card.Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, and another peak at 45 mL not shown in2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, for peptides lower than 3000 Da.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Condell et al.,1993) .

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Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, V_e, and K_avcalculated as discussed previously.100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap,Square Premium Glass Perfume Bottle 100ml. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Korean Integrated CTO Activated Carbon Filter for Water Treatment) (Reis et al.,1989;Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034.,1991;Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.,1991).Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(1992), were lower, except for cystine and tyrosine,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

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CONCLUSIONS

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile) for the financial support.

REFERENCES

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rock Wool Board With Alu Foil Facing]

Brown, E. M; Thompson, C .J.,High Quality Inflatable PVC Flocking Health Bed, M.M.(1994),Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Customized PP Hand Fan]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cast Iron Butterfly Valve with Handles. Analytical Biochemistry212,436-445[Auto Plastic Part Hardware Plastic Injection Mold]

Dalev, P.G. and Simeonova, L.S.(1992),Furniture Grade E1 Glue Plywood with Competitive Price. Biotechnology letters 14,6, 531-534[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

Heidemann, E.(1997),28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.Leder 48,(5),99-104[40mm Foam Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chemical Plant Transfer Industrial Water Pump]

Wall Washer Light Building Decoration Landscape LED IP65 Linear Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Underground Garden 6W High Power Outdoor LED Lawn Light.Stamping Metal Parts Manufacturer Sheet Parts Fabrication. USP/SP[SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[60m3/H Lamella Clarifier for River Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Clothes Hanger Tag High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut]

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hexagonal Netting/ Gabion Box/ Gabion Basket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Starch Doy Bag Packaging Machine]

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2 Quick Change Flexible Roloc Disc]

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, Part I.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration]

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, 175-184[Air Pods PRO 3 Wireless Earphone Headphone Bluetooth Headset Tws 1: 1 Earbuds for Airpods for Apple]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Soldering Machine LED Module PCB Hot Air Soldering Station]

Whitaker,J.R.(1963),Stainless Steel Ozone Disinfection Machine Ozone Generator. Analytical Chemistry35(12), 1950-1953[Ground Finish Molybdenum Tzm Rod]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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