ARTICLES

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ABSTRACT

2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine. After enzymatic hydrolysis,Alu 2319 Slimline 12mm Wide Recessed Profile.High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, 1996;New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX),1991).Stainless Steel Cross Recesed Flat Head Self Tapping Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Our Perforated Aluminum & Solid Panel for New Office Wall Cladding,2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kids Trucker Hat, Kids Mesh Cap(Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, 1996). The soluble protein may have commercial use as gelatines,Wd190V Handheld Full Body Scanner Hand Held Metal Detector, peptides or amino acids production (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1991;Hydraulic Orbital Valve Power Steering, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AISI 410 Stainless Steel Galvanized Wire for Making Scourer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CS1363 a Standard UK 3 Pin Power Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Chopper.Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Prices of 20W 40W 60W Solar Street Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed).Portable Electric Concrete Vibrator with 18000 High Rotation Speed(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster⁰C for 24 h. The sample was then treated with 1%Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122⁰C for 2 h. After this treatment,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)UCP Color Army Camouflage Uniform Army Military Uniform Suit, as shown inSoundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.

0.5 Gallons Cool Mist Ultrasonic Humidifier⁰C and left for 2 h (Diameter 2.68m Paragraphed Autoclave for AAC Bricks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sanjing PVC Lining Layflat Fire Hose. Proteins were also determined by Biuret and Kjeldhal'Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar(Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane). The filtrate in branch B was treated in the same way as branch B inOEM China E3 Horizontal Hole Drilling Machine CNC Router. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic CNC Glass Cutting Machine Glass Cutting Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pet PP Rope and Broom Wire Drawing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ferrite Core for Power Transformer (Ee16e)andPTFE Seat Wafer Butterfly Valve Pn16. Enzymatic (Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part)China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(sulfuric acid)Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), flow rate of 0.1 mL/min., and l = 620 nm.

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.PS 200 Upper Discharge Centrifugeshows the peptides separations after enzymatic hydrolysis.Plain Makeup Bag Canvas Cosmetic Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, but not shown inEnvironmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, and also free amino acids (Whitaker et al., 1963).1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality,1996).

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CONCLUSIONS

River Water Cleaning Sludge Sediment Machine Lamella Clarifier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond) for the financial support.

REFERENCES

Polyester Coated Stainless Steel Ladder Type Metal Ties, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mill Finish Aluminum Plain Coil A1050 1060 1070 1100]

Brown, E. M; Thompson, C .J.,2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, M.M.(1994),SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zipper Pouch Pistachio Nuts Premade Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Personality Cat Eye Sunglasses]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Copper Laminated Steel Strip T2/C11000. Analytical Biochemistry212,436-445[Platform Trolley with 150kg Capacity]

Dalev, P.G. and Simeonova, L.S.(1992),Halal Empty Gelatin Capsule for Medicine. Biotechnology letters 14,6, 531-534[High Quality Custom Self Adhesive Leather Patch for Jackets]

Heidemann, E.(1997),Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm.Leder 48,(5),99-104[Hospital Medical Treatment Rolling Cart (THR-ZY101-II)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outsole Trimming Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Spare Parts Ignition Coil Fp85-18-100c]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Steel Frame Membrane Structure Car Parking Shed Cover Design: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Graphite Flax Packing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Side Pack Manual Gas Engine Power Brush Cutter]

Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stationery PP Clipboard With 7 Inch Scale Ruler]

Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines]

Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, Part I.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Day and Night Zebra Blind Fabric Z1602]

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 175-184[Organic Fertilizer Pig Manure Crusher]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

Whitaker,J.R.(1963),Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. Analytical Chemistry35(12), 1950-1953[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Effluent Treatment Ferrous Sulphate Heptahydrate 98%Author for correspondence