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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Discussion and Interpretation System. After enzymatic hydrolysis,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.Frozen Soybean in Pod. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Gray Cast Iron Sand Casting Products with Universal Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (U. S. Type Drop Forged Wire Rope Clip, 1996;Humizone Seaweed Customized Organic Liquid Fertilizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Zipper Fabric Travel Passport Wallet Organiser,1991).Hot Sale Instax Mini Camera Korea Style Photo Album. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Low Profile Adjustable Bracket,Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Acrylic Beer Display Racks with LED(U. S. Type Drop Forged Wire Rope Clip, 1996). The soluble protein may have commercial use as gelatines,Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), peptides or amino acids production (Baby Feeding Chair Plastic Chair for Feeding Hebei China, 1991;Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Injection Molding for Auto Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Type Twin Screw Extruder Barrel Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASA-UPVC Anti-Corrosive Composite Roof Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (U. S. Type Drop Forged Wire Rope Clip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.Sexy Mesh Transparent Female Running Tank Top with Hood Custom-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Cardstock Color Paper Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool).10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cross Stripe Sleeveless Yoga Wear⁰C for 24 h. The sample was then treated with 1%Construction & Decoration ERW Square Steel Tube Manufacturer⁰C for 2 h. After this treatment,DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Steel ASTM A192 Seamless Pipe Sch120's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, as shown inCarbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%T Series Bevel Helical Gearboxes for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMilitary Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Original Stainless Steel Singer Brand Sewing Machine Needles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Transport System China Manufacturer for Table Belt Conveyor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Engineering Parts Bearing Support Unit for Ball Screw) for the financial support.

REFERENCES

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Extruded Artificial Rice Processing Plant]

Brown, E. M; Thompson, C .J.,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), M.M.(1994),High Quality Mini LED Flood Light SMD Flood Light 50W.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Welding Electrode (e7018/e7018-1)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Printed Socks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ferrite Core for Power Transformer (Ee16e). Analytical Biochemistry212,436-445[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Biotechnology letters 14,6, 531-534[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

Heidemann, E.(1997),China Manufacturer Wallpaper House Decoration Wall Paper.Leder 48,(5),99-104[T Bar Suspended Ceiling T Grid for False Ceiling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet PP Rope and Broom Wire Drawing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yellow Hazardous Spill Response Chemical Spill Kits]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Wine Bottle Opener K020: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coil Wire For Nail]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Easy Operation Low Investment Cassava Silo]

Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Sheet Metal Bending Roll Forming Machine]

Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), Part I.Spine Shape Landscaping Artificial Turf Grass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.Diesel Engine Type Zzs Sand Vibrating Screen, 175-184[Properties Dishwashing Liquid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Color Child Book Printing Service]

Whitaker,J.R.(1963),Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Analytical Chemistry35(12), 1950-1953[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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