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ASME VIII Pressure Vessel for Curing Carbon Fiber Parts
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
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ARTICLES
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate
ABSTRACT
Discussion and Interpretation System. After enzymatic hydrolysis,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.Frozen Soybean in Pod. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Custom Sheet Metal Stamping Bending Product Manufacturing Service;Waterproof EVA Rubber Roofing Material for Shingle Building; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.OEM Gray Cast Iron Sand Casting Products with Universal Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (U. S. Type Drop Forged Wire Rope Clip, 1996;Humizone Seaweed Customized Organic Liquid Fertilizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Zipper Fabric Travel Passport Wallet Organiser,1991).Hot Sale Instax Mini Camera Korea Style Photo Album. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Low Profile Adjustable Bracket,Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Acrylic Beer Display Racks with LED(U. S. Type Drop Forged Wire Rope Clip, 1996). The soluble protein may have commercial use as gelatines,Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), peptides or amino acids production (Baby Feeding Chair Plastic Chair for Feeding Hebei China, 1991;Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Injection Molding for Auto Parts., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Type Twin Screw Extruder Barrel Manufacturer.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASA-UPVC Anti-Corrosive Composite Roof Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (U. S. Type Drop Forged Wire Rope Clip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.Sexy Mesh Transparent Female Running Tank Top with Hood Custom-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Cardstock Color Paper Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare PartsAlcalase2.5 L DX,Home and Office PVC Vinyl Flooring Conductive Plank Flooring, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Precision Engineering Parts Bearing Support Unit for Ball Screw); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, Parana State,New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate.Silicone Cake Mold Sinle Cupcake DIY, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (U. S. Type Drop Forged Wire Rope Clip, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLatex Paint Emulsion Thickener Agents with High Leveling Propertyandvacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment.
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Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool).10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cross Stripe Sleeveless Yoga Wear0C for 24 h. The sample was then treated with 1%Construction & Decoration ERW Square Steel Tube Manufacturer0C for 2 h. After this treatment,DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Steel ASTM A192 Seamless Pipe Sch120's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, as shown inCarbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.
Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine0C and left for 2 h (Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. Proteins were also determined by Biuret and Kjeldhal'CNC Machining Checking Auto Car Checking Fixture(Humizone Seaweed Customized Organic Liquid Fertilizer). The filtrate in branch B was treated in the same way as branch B inOEM High Precision Custom Automotive Spare Parts CNC Machining Car Parts Sheet Metal Stamping Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%T Series Bevel Helical Gearboxes for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavMilitary Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Original Stainless Steel Singer Brand Sewing Machine Needles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Baby Finger ToothbrushandSilicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance. Enzymatic (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve)Steel Bar Peeling Lathe Machine(sulfuric acid)Chemical Product Sodium Gluconate Used for Concrete Admixture(U. S. Type Drop Forged Wire Rope Clip, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Adding Water Rice Pulping Machine/Soybean Grinding Machine, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Adding Water Rice Pulping Machine/Soybean Grinding Machine, flow rate of 0.1 mL/min., and l = 620 nm.
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Black Steel ASTM A192 Seamless Pipe Sch120andSexy Mesh Transparent Female Running Tank Top with Hood Customshow the gel chromatographic separation of soluble protein with Sephadex G-Aluminum Mushroom Rround Head Solid Rivet (Round head).Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, and another peak at 45 mL not shown inKorean Style PE Foam 3D Wallpapers, for peptides lower than 3000 Da.Warm Women Suede Leisure Snow Boots for Winter (FF-1)(Condell et al.,1993) .
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CONCLUSIONS
Transport System China Manufacturer for Table Belt Conveyor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Engineering Parts Bearing Support Unit for Ball Screw) for the financial support.
REFERENCES
Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Extruded Artificial Rice Processing Plant]
Brown, E. M; Thompson, C .J.,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), M.M.(1994),High Quality Mini LED Flood Light SMD Flood Light 50W.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Welding Electrode (e7018/e7018-1)]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Printed Socks]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ferrite Core for Power Transformer (Ee16e). Analytical Biochemistry212,436-445[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]
Dalev, P.G. and Simeonova, L.S.(1992),Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Biotechnology letters 14,6, 531-534[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]
Heidemann, E.(1997),China Manufacturer Wallpaper House Decoration Wall Paper.Leder 48,(5),99-104[T Bar Suspended Ceiling T Grid for False Ceiling]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet PP Rope and Broom Wire Drawing Machine]
380mm Working Area Concrete Diamond Floor Edge Grinder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Flexible Magnetic Rubber Sheet Soft Magnetic Tape.Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. USP/SP[100% Acrylic Knitted Sports Woven Scarf]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yellow Hazardous Spill Response Chemical Spill Kits]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Wine Bottle Opener K020: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coil Wire For Nail]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Easy Operation Low Investment Cassava Silo]
Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Sheet Metal Bending Roll Forming Machine]
Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]
Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), Part I.Spine Shape Landscaping Artificial Turf Grass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]
08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.Diesel Engine Type Zzs Sand Vibrating Screen, 175-184[Properties Dishwashing Liquid]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Color Child Book Printing Service]
Whitaker,J.R.(1963),Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Analytical Chemistry35(12), 1950-1953[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
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