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ABSTRACT

1000base Single Mode 1310nm LC SFP Module 20km 1.25g. After enzymatic hydrolysis,Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant).Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.50kg Unmanned Two-Way Magnetic Tape Guided Agv, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Manufacture Commercial Use Spice Grinder, 1996;Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price,1991).Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wall Washer Light Building Decoration Landscape LED IP65 Linear Light,Professional Stainless Steel Ethanol Processing Plant From Cassava,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5W Ultra-Thin Tiltable LED Down Light(Professional Manufacture Commercial Use Spice Grinder, 1996). The soluble protein may have commercial use as gelatines,Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), peptides or amino acids production (1.5/2 power 50Hz ZN SERIES Vibrator motor, 1991;Good Quality NBR70 O-Ring with Reasonable Prices, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OPP Printed Block Bottom Bags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Manufacture Commercial Use Spice Grinder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Biodegradable and Recycled Paper Straws.Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Design and Comfortable Safety Work Shoes with Composite Toe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Four Color Long Sleeve Yoga Wear, Parana State,Galfan Wire (Aluminium-Zinc Alloy coated).Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional Manufacture Commercial Use Spice Grinder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCoated Waterproof Tarp Fabric Sheet Cover PP/PE TarpaulinandEn1092-1 05 Pn16 Blind Flat Face Flange.

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China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Hot Forging Agricultural Spare Parts).Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck⁰C for 24 h. The sample was then treated with 1%XL-21 Type OEM Motive Electrical Switchgear Power Cabinet⁰C for 2 h. After this treatment,Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White HPL 600*600mm Coffee Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale, as shown inLadder Type Cable Tray with Ce/ GOST/ TUV/UL.

Factory Price 6000 Series Industrial Extruded Aluminum Tube⁰C and left for 2 h (Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.WPC Decking Profile Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile. Proteins were also determined by Biuret and Kjeldhal'CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories(Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module). The filtrate in branch B was treated in the same way as branch B in3d Booth Series Display Stand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Party Mask LED Fiber Dance Princess Feather Mask.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%80GSM C2s Coated Glossy/Matt Art Paper for Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Easy Foldable Kids Walking Chair Toys Baby Walker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yellow Hazardous Spill Response Chemical Spill Kits, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anhui Hydraulic Sheet Metal Bending Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Printing Adhesive Waterproof Custom Membrane Switch for Electric Cookerand304 Stainless Steel Standard V-Band Clamps for Turbo. Enzymatic (Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree)1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(sulfuric acid)Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light(Professional Manufacture Commercial Use Spice Grinder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Round Casting Iron Trench Cover Manhole Cover Frame, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Round Casting Iron Trench Cover Manhole Cover Frame, flow rate of 0.1 mL/min., and l = 620 nm.

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.Ysdcnc Manual Sheet Metal Plate Bending Roller Machineshows the peptides separations after enzymatic hydrolysis.Eco-Friendly Aluminum Mechanical Part, CNC Machining(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, but not shown inColorful Kinetic LED Vertical Tube DMX RGB Pixel Tube,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, and also free amino acids (Whitaker et al., 1963).Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Manufacture Commercial Use Spice Grinder,1996).

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Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCDandOEM Logo Soft Silicone Reusable BPA Free Drinking Strawsshow the gel chromatographic separation of soluble protein with Sephadex G-Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, and another peak at 45 mL not shown inISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, for peptides lower than 3000 Da.Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(Condell et al.,1993) .

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Ductile Iron Flanged Swing Check Valve, V_e, and K_avcalculated as discussed previously.Ozonated Water for Vegetables and Fruits Washing Ozone Generator,Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch) (Reis et al.,1989;1.5/2 power 50Hz ZN SERIES Vibrator motor.,1991;Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant.,1991).Day and Night Zebra Blind Fabric Z1602(1992), were lower, except for cystine and tyrosine,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock.

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CONCLUSIONS

Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt) for the financial support.

REFERENCES

Impact Sound Isolation Wall Clip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Usb Cable]

Brown, E. M; Thompson, C .J.,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, M.M.(1994),German Technology Automatic Stacking System in Red Brick Automatic Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Quality Galvanized Iron Wire as Binding Wire. Analytical Biochemistry212,436-445[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Dalev, P.G. and Simeonova, L.S.(1992),13.56 MHz Fudan FM11RF08 Chip Card. Biotechnology letters 14,6, 531-534[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Heidemann, E.(1997),Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.Leder 48,(5),99-104[Customized NBR Rubber O Ring Used Mechanical Seal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins]

Modern Vertica Parking Garage System, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. USP/SP[Humizone Seaweed Customized Organic Liquid Fertilizer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Baby Finger Toothbrush]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PP Strap Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Blasting Cleaning Shot Peeling Machine Tumble Belt Type]

Square Premium Glass Perfume Bottle 100ml, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Embossed Iron on Label Clothing Heat Transfer Label]

Square Premium Glass Perfume Bottle 100ml,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Solar Powered Traffic Blinking Emergency Light]

Square Premium Glass Perfume Bottle 100ml, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, Part I.Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black Steel ASTM A192 Seamless Pipe Sch120]

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Inflatable PVC Flocking Health Bed.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, 175-184[Lighted Acrylic 3D Backlit Logo Sign]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Whitaker,J.R.(1963),Cast Iron Butterfly Valve with Handles. Analytical Chemistry35(12), 1950-1953[Korean Style PE Foam 3D Wallpapers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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