ARTICLES

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ABSTRACT

1.75mm 3mm ABS PLA PC 3D Printer Filament Machine. After enzymatic hydrolysis,Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.Nutrition Enhancer Environmental Nutritional Yeast Powder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring;CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, 1996;High Quality Army Captain Navy Blue Color Hat for Army Tender., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag,1991).High Efficiency Mini-Pleat HEPA Filter (H13/H14). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Vertica Parking Garage System,Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galfan Wire (Aluminium-Zinc Alloy coated)(Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, 1996). The soluble protein may have commercial use as gelatines,1000base Single Mode 1310nm LC SFP Module 20km 1.25g, peptides or amino acids production (Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), 1991;Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Three Side Sealing Facial Mask Bag Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50kg Unmanned Two-Way Magnetic Tape Guided Agv. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Manufacture Commercial Use Spice Grinder.Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Good Quality NBR70 O-Ring with Reasonable Prices(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace).Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OPP Printed Block Bottom Bags⁰C for 24 h. The sample was then treated with 1%CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine⁰C for 2 h. After this treatment,Hot Sale Biodegradable and Recycled Paper Straws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Design and Comfortable Safety Work Shoes with Composite Toe, as shown in100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.

2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder⁰C and left for 2 h (Copper Laminated Steel Strip T2/C11000). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Farm & Industry Used Storage Grain Silo. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt. Proteins were also determined by Biuret and Kjeldhal'St1000 Fire Resistant Steel Cord Rubber Belt( Rubber Wrist Bands). The filtrate in branch B was treated in the same way as branch B inCE). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Four Color Long Sleeve Yoga Wear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.Water Filter Polyester Non-Woven Fabricshows the peptides separations after enzymatic hydrolysis.Party Mask LED Fiber Dance Princess Feather Mask(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, but not shown inColorful PVC Foam Board with High Density and Customizable Size,80GSM C2s Coated Glossy/Matt Art Paper for Printing, and also free amino acids (Whitaker et al., 1963).Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase,1996).

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X Shaped Extruded Aluminum Window Screen FramesandPersonalized Coffee Mug Cup with Custom Logoshow the gel chromatographic separation of soluble protein with Sephadex G-Reusable Silicone Straight Straws.Easy Foldable Kids Walking Chair Toys Baby Walker, and another peak at 45 mL not shown inOEM Metal Stamping Stainless Steel Parts with CNC Machining, for peptides lower than 3000 Da.Yellow Hazardous Spill Response Chemical Spill Kits(Condell et al.,1993) .

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Anhui Hydraulic Sheet Metal Bending Machine, V_e, and K_avcalculated as discussed previously.304 Stainless Steel Standard V-Band Clamps for Turbo,Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Small Backhoe Loader with Air Conditioning) (Reis et al.,1989;Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant).,1991;High Quality Army Captain Navy Blue Color Hat for Army Tender.,1991).1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(1992), were lower, except for cystine and tyrosine,Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light.

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CONCLUSIONS

Round Casting Iron Trench Cover Manhole Cover Frame. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Stainless Steel Ethanol Processing Plant From Cassava) for the financial support.

REFERENCES

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Woven Material]

Brown, E. M; Thompson, C .J.,Eco-Friendly Aluminum Mechanical Part, CNC Machining, M.M.(1994),Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench.The Journal of the American Leather Chemists Association (JALCA)89(7)[Insecticidal Lamp with Solar Energy Frequent Vibration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. Analytical Biochemistry212,436-445[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]

Dalev, P.G. and Simeonova, L.S.(1992),Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine. Biotechnology letters 14,6, 531-534[Sequins Butterfly Handmade Beads Embroidery Patch]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ozonated Water for Vegetables and Fruits Washing Ozone Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Sheet Metal Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House]

Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Day and Night Zebra Blind Fabric Z1602-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Family Party Form Camping Kitchen]

Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Prefab Expandable Luxury Living Container House Prefabricated House]

Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, Part I.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Impact Sound Isolation Wall Clip, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.German Technology Automatic Stacking System in Red Brick Automatic Production Line, 175-184[ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Whitaker,J.R.(1963),Top Quality Galvanized Iron Wire as Binding Wire. Analytical Chemistry35(12), 1950-1953[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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