ARTICLES

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ABSTRACT

Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. After enzymatic hydrolysis,0.1mm Tungsten Carbide 2f Single Flute Micro End Mill.Agriculture Large Span Commerical Steel Frame Greenhouse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, 1996;Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chinese Jbcz Handy Operation Threading Machine and Coupler,1991).Promotional Reusable Foldable PP Non Woven Shopping Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board,Factory Price Printing Doy Pack Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment(High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, 1996). The soluble protein may have commercial use as gelatines,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, peptides or amino acids production (Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, 1991;High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Engine Parts Rubber High Quality Timing Belt 111ru25., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Interior Metal 3D Bee Garden Ornament Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Non-Woven Dry Gloves Wipes Making Machine.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree⁰C and left for 2 h (16mm 12 Strand UHMWPE Synthetic Fiber Rope). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. Proteins were also determined by Biuret and Kjeldhal'Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation(Ozonated Water for Vegetables and Fruits Washing Ozone Generator). The filtrate in branch B was treated in the same way as branch B inThickness 0.1mm to 0.2mm Copolymer Copper Tape for Wire and Cable Armouring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%125kHz RFID Hotel Key Card with T5577 Chip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUSB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Crop Fertilizer Agriculture Nh4cl Ammonium Chloride⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale High Pressure 80L Nitrogen Gas Cylinder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/10mm Red Green Yellow Warning China Supplier Signal Lamp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Diamond Segments 2000mm Diameter Granite Cutting Blade) for the financial support.

REFERENCES

Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Extruder Granulating Machine]

Brown, E. M; Thompson, C .J.,Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, M.M.(1994),High Strength Rigid PVC Plastic Foam Board for FRP.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flooded Type Water Cooled Screw Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. Analytical Biochemistry212,436-445[Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape]

Dalev, P.G. and Simeonova, L.S.(1992),4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Biotechnology letters 14,6, 531-534[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Heidemann, E.(1997),Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.Leder 48,(5),99-104[Cement Grouting Pump Gun]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Design and Nice Price All in One Solar Street Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eggs Paper Machine Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics]

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner]

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, Part I.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Aluminum Sheet Press Brake]

Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Ceramic Chrome Hybrid Bearing Oil.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, 175-184[Deluxe Outdoor Pool]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed]

Whitaker,J.R.(1963),CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. Analytical Chemistry35(12), 1950-1953[High Quality Step Motor for Intelligent Sanitary/3D Printer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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