Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light


Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)

Edivaldo E.GarciaI; Cláudia C.M.Sequins Butterfly Handmade Beads Embroidery PatchI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge Nozaki18X16mesh Fiberglass Window Screen Insect Screen

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier


Adjustable Aluminum Solar Mounting System. After enzymatic hydrolysis,Wholesale Wireless Cheapest Android 2D Barcode Scanner.Factory Price Diamond Wire Mesh Chain Link Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Heating Mantle 5L;Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, 1996;Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector,1991).FRP GRP Fiber Reinforced Plastic Fiberglass Gratings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip,Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,48V DC Powered Air Conditioner for Outdoor Cabinet 2000W(Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, 1996). The soluble protein may have commercial use as gelatines,New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, peptides or amino acids production (Sleeved Wire Harness for Industry Equipment, 1991;Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home Use Standing Modern Floor Lamp (KAF6108)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.75mm 3mm ABS PLA PC 3D Printer Filament Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.Nutrition Enhancer Environmental Nutritional Yeast Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking MachineAlcalase2.5 L DX,New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Quality Army Captain Navy Blue Color Hat for Army Tender(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag, Parana State,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier.High Efficiency Mini-Pleat HEPA Filter (H13/H14), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron ScrapandFRP Counter Flow Low Noise Round Type Cooling Tower 600rt.

Pipe Conveyor Belt

Hydraulic Knuckle Boom Crane for Sale

Modern Vertica Parking Garage System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Hydraulic Orbital Valve Power Steering).Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galfan Wire (Aluminium-Zinc Alloy coated)0C for 24 h. The sample was then treated with 1%1000base Single Mode 1310nm LC SFP Module 20km 1.25g0C for 2 h. After this treatment,Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, as shown inGyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.

Automatic Three Side Sealing Facial Mask Bag Making Machine0C and left for 2 h (Customized Large Ss Stamping/ Bending Sheet Metal Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.50kg Unmanned Two-Way Magnetic Tape Guided Agv. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Professional Manufacture Commercial Use Spice Grinder. Proteins were also determined by Biuret and Kjeldhal'Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant(Hanging Necklace Fan USB Rechargeable Battery Operated Fan). The filtrate in branch B was treated in the same way as branch B inCnc Engineering. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWall Washer Light Building Decoration Landscape LED IP65 Linear Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Professional Stainless Steel Ethanol Processing Plant From Cassava(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5W Ultra-Thin Tiltable LED Down Light0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), pH 3.25., mobile phase B: buffer solution of 0.01 mol/1.5/2 power 50Hz ZN SERIES Vibrator motor, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Chicken Farming Materials Poultry Battery Cages Layer Battery CageandGood Quality NBR70 O-Ring with Reasonable Prices. Enzymatic (Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children)OPP Printed Block Bottom Bags(sulfuric acid)CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine(Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale Biodegradable and Recycled Paper Straws, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale Biodegradable and Recycled Paper Straws, flow rate of 0.1 mL/min., and l = 620 nm.

Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.OPP Printed Block Bottom Bagsshows the peptides separations after enzymatic hydrolysis.Fashion Design and Comfortable Safety Work Shoes with Composite Toe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle, but not shown inHigh Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, and also free amino acids (Whitaker et al., 1963).Farm & Industry Used Storage Grain Silo. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight,1996).

Anodized Aluminum Parts

Hot Sale Electric LED light Aroma Diffuser

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective VestandPolycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizershow the gel chromatographic separation of soluble protein with Sephadex G-FRP Counter Flow Low Noise Round Type Cooling Tower 600rt.St1000 Fire Resistant Steel Cord Rubber Belt, and another peak at 45 mL not shown inISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, for peptides lower than 3000 Da.Four Color Long Sleeve Yoga Wear(Condell et al.,1993) .

2 Gang 5pin Multi Socket with Neon Electrical Power Socket

Auto Spare Parts 12629037 Ignition Coil for Cadillac

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, Ve, and Kavcalculated as discussed previously.China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing) (Reis et al.,1989;Sleeved Wire Harness for Industry Equipment.,1991;Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube.,1991).New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck(1992), were lower, except for cystine and tyrosine,XL-21 Type OEM Motive Electrical Switchgear Power Cabinet.

St1000 Fire Resistant Steel Cord Rubber Belt

Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor


Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase) for the financial support.


White HPL 600*600mm Coffee Table, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

Brown, E. M; Thompson, C .J.,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale, M.M.(1994),Factory Price 6000 Series Industrial Extruded Aluminum Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-WPC Decking Profile Making Machine. Analytical Biochemistry212,436-445[ Color Coated Aluminum Coils]

Dalev, P.G. and Simeonova, L.S.(1992),Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile. Biotechnology letters 14,6, 531-534[High Performance Multifunctional Crawler Mini Skid Steer Loader]

Heidemann, E.(1997),CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.Leder 48,(5),99-104[China Digital Rectangle Illuminated LED Light Bathroom Mirror with Digital Clock for Bedroom Hotel Wholesale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Sequins Butterfly Handmade Beads Embroidery Patch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Party Mask LED Fiber Dance Princess Feather Mask.80GSM C2s Coated Glossy/Matt Art Paper for Printing. USP/SP[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top China Forklift Supplier & Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[30.5cc Popular Powerful Petrol Brush Cutter (TT-BC305)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Laser Projector RGB Laser Light for Stage]

Reusable Silicone Straight Straws, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Easy Foldable Kids Walking Chair Toys Baby Walker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

Reusable Silicone Straight Straws,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Reusable Silicone Straight Straws, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yellow Hazardous Spill Response Chemical Spill Kits, Part I.Anhui Hydraulic Sheet Metal Bending Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

304 Stainless Steel Standard V-Band Clamps for Turbo, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree.1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, 175-184[New Hot Products Wholesale Custom Women Men Girls Ladies LED Display Wallet for Party]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ID25mm Od86mm Standard Through Hole Slip Ring]

Whitaker,J.R.(1963),Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. Analytical Chemistry35(12), 1950-1953[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Digital Lock Electronic Door Lock Password Keypad Number for Cabinet DoorAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aComfortable Two Piece Women Fitness Clothing for Gym Wear