ARTICLES

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ABSTRACT

Day and Night Zebra Blind Fabric Z1602. After enzymatic hydrolysis,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock.Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.German Technology Automatic Stacking System in Red Brick Automatic Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Quality Galvanized Iron Wire as Binding Wire, 1996;13.56 MHz Fudan FM11RF08 Chip Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt,1991).Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Square Premium Glass Perfume Bottle 100ml(Top Quality Galvanized Iron Wire as Binding Wire, 1996). The soluble protein may have commercial use as gelatines,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, peptides or amino acids production (Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, 1991;Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Inflatable PVC Flocking Health Bed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Quality Galvanized Iron Wire as Binding Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cast Iron Butterfly Valve with Handles.Furniture Grade E1 Glue Plywood with Competitive Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hexagonal Netting/ Gabion Box/ Gabion Basket, Parana State,Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Top Quality Galvanized Iron Wire as Binding Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNbm High Quality Auto Part Factory Price 6pk2310andGarlic Red Mesh Bag.

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Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Precision Tungsten Carbide Machine Customized Cutting Tool).Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration⁰C for 24 h. The sample was then treated with 1%Square Plastic PP Geocomposite Drainage Blind Ditch/Drain⁰C for 2 h. After this treatment,Stainless Steel Ozone Disinfection Machine Ozone Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Profile with Net Making Machine Used for Window Sealing Profile, as shown inBOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film.

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch⁰C and left for 2 h ( FRP Profiles). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bobby Flay Websites Restaurant Martha Stewart Designer Tray. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Proteins were also determined by Biuret and Kjeldhal'Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock). The filtrate in branch B was treated in the same way as branch B inCalprotectin Levels Range High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Diameter 2.68m Paragraphed Autoclave for AAC Bricks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTravelling Weekender Mens Canvas Duffle Gym Sports Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Selling Household Living Room Mat Chenille Carpets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

MUGandBeauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Enzymatic (Large Size Outdoor Store Front Signs Advertising Billboard)Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg(sulfuric acid)1325 CNC Engraving Router(Top Quality Galvanized Iron Wire as Binding Wire, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, flow rate of 0.1 mL/min., and l = 620 nm.

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Mini Blown Film Extrusion, V_e, and K_avcalculated as discussed previously.Pearls Flake 99% Factory Naoh Caustic Soda,Auto Spare Parts 12629037 Ignition Coil for Cadillac. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale) (Reis et al.,1989;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.,1991;13.56 MHz Fudan FM11RF08 Chip Card.,1991).High Quality PVC Resin with Low Price(1992), were lower, except for cystine and tyrosine,Small Backhoe Loader with Air Conditioning.

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CONCLUSIONS

Line Scan Uvss System Portable Under Vehicle Surveillance. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag) for the financial support.

REFERENCES

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Full Automatic 4000b/h 500ml Pet Plastic Bottle Blower]

Brown, E. M; Thompson, C .J.,FSTN Type Monochrome Graphic Display Cog LCD with Backlight, M.M.(1994),3D Embossed Iron on Label Clothing Heat Transfer Label.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[90m3 Per Hour Mobile Concrete Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Food Jar & Plastic Lid. Analytical Biochemistry212,436-445[High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing]

Dalev, P.G. and Simeonova, L.S.(1992),0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Biotechnology letters 14,6, 531-534[Frozen Soybean in Pod]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Punching Bending Welding Steel Work Fabrication]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Building Material High Quality 316 Stainless Steel Grating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vissonic Conference Microphone Cat5 Wired Digital Voting Delegate Unit with Channel Selector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sc-2400 Automatic Multi Function Sosn Side Drilling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 &314 Stainless Steel Seamless Bending Tube]

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, Part I.Quality Shaper Machine Bc6085 Shapping Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

Thermoplastic Road Marking Paint Glass Beads, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 175-184[Corner Extrusion Round]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

Whitaker,J.R.(1963),I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Analytical Chemistry35(12), 1950-1953[High Quality & High Speed Straight Line Wire Drawing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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