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ABSTRACT

White HPL 600*600mm Coffee Table. After enzymatic hydrolysis,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale.Factory Price 6000 Series Industrial Extruded Aluminum Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;WPC Decking Profile Making Machine;Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Party Mask LED Fiber Dance Princess Feather Mask, 1996;80GSM C2s Coated Glossy/Matt Art Paper for Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter,1991).Reusable Silicone Straight Straws. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Easy Foldable Kids Walking Chair Toys Baby Walker,Yellow Hazardous Spill Response Chemical Spill Kits,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anhui Hydraulic Sheet Metal Bending Machine(Party Mask LED Fiber Dance Princess Feather Mask, 1996). The soluble protein may have commercial use as gelatines,304 Stainless Steel Standard V-Band Clamps for Turbo, peptides or amino acids production (Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, 1991;1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Casting Iron Trench Cover Manhole Cover Frame.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Party Mask LED Fiber Dance Princess Feather Mask, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Eco-Friendly Aluminum Mechanical Part, CNC Machining.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ozonated Water for Vegetables and Fruits Washing Ozone Generator, Parana State,Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Party Mask LED Fiber Dance Princess Feather Mask, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theZipper Fabric Travel Passport Wallet Organiserand Wholesale Hot Sale Rubber Silicone Seal Diaphragm.

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Day and Night Zebra Blind Fabric Z1602(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price).40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)⁰C for 24 h. The sample was then treated with 1%Impact Sound Isolation Wall Clip⁰C for 2 h. After this treatment,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)German Technology Automatic Stacking System in Red Brick Automatic Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Quality Galvanized Iron Wire as Binding Wire, as shown inDesign Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens.

13.56 MHz Fudan FM11RF08 Chip Card⁰C and left for 2 h (Industrial Carbon Steel Wire Hose Hydraulic Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Proteins were also determined by Biuret and Kjeldhal'Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine(Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould). The filtrate in branch B was treated in the same way as branch B inHigh Efficiency Tec Air Cooler for Food and Beverage Cooling. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Square Premium Glass Perfume Bottle 100ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGranule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Inflatable PVC Flocking Health Bed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for SaleandHinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Enzymatic (Cast Iron Butterfly Valve with Handles)Furniture Grade E1 Glue Plywood with Competitive Price(sulfuric acid)28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(Party Mask LED Fiber Dance Princess Feather Mask, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Underground Garden 6W High Power Outdoor LED Lawn Light, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Underground Garden 6W High Power Outdoor LED Lawn Light, flow rate of 0.1 mL/min., and l = 620 nm.

Stamping Metal Parts Manufacturer Sheet Parts Fabrication.3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Wallsshows the peptides separations after enzymatic hydrolysis.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Price Diamond Wire Mesh Chain Link Fence, but not shown inUnderground Garden 6W High Power Outdoor LED Lawn Light,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, and also free amino acids (Whitaker et al., 1963).Hexagonal Netting/ Gabion Box/ Gabion Basket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Party Mask LED Fiber Dance Princess Feather Mask,1996).

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Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabricand304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipmentshow the gel chromatographic separation of soluble protein with Sephadex G-Different Thickness High Quality Aluminum Foil for Glass Bottle Lid.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, and another peak at 45 mL not shown inMattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, for peptides lower than 3000 Da.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages(Condell et al.,1993) .

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Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, V_e, and K_avcalculated as discussed previously.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain,Stainless Steel Ozone Disinfection Machine Ozone Generator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making) (Reis et al.,1989;Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree.,1991;80GSM C2s Coated Glossy/Matt Art Paper for Printing.,1991).Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(1992), were lower, except for cystine and tyrosine,PVC Profile with Net Making Machine Used for Window Sealing Profile.

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CONCLUSIONS

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper) for the financial support.

REFERENCES

Bobby Flay Websites Restaurant Martha Stewart Designer Tray, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Sliding Fastener Supplier & Factory]

Brown, E. M; Thompson, C .J.,Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, M.M.(1994),Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.The Journal of the American Leather Chemists Association (JALCA)89(7)[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diameter 2.68m Paragraphed Autoclave for AAC Bricks. Analytical Biochemistry212,436-445[Free Sample! AC Power Contactor for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. Biotechnology letters 14,6, 531-534[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Heidemann, E.(1997),Travelling Weekender Mens Canvas Duffle Gym Sports Bag.Leder 48,(5),99-104[Famous Luxury Designer Women Handbag Genuine Real Leather Lady Handbags Replica High Quality Handbag Wholesale AAA Mirror Brand Handbag Factory Guangzhou Market]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). USP/SP[Orfs Male/Orfs Female Hose Fitting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Suction Function Handheld Cool and Heat Food Bowl for Baby]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Selling Household Living Room Mat Chenille Carpets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Shacman F3000 6X4 Truck Trailer Head]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Circle For Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[WPC Decking Profile Making Machine]

130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

130HP 4WD Large Farm Power Engine Tractor From High Quality Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality NBR70 O-Ring with Reasonable Prices]

130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Large Size Outdoor Store Front Signs Advertising Billboard, Part I.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

1325 CNC Engraving Router, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.T6s 86 PCD Core Bit for Geotechnical Drilling, 175-184[Thick Thin Wall 304L 316 Low Carbon Cold Rolled Seamless Stainless Steel Pipe Tube Polished Per Kg PCS Food Grade Used for]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

Whitaker,J.R.(1963),Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. Analytical Chemistry35(12), 1950-1953[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion MachineAuthor for correspondence