ARTICLES

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ABSTRACT

Colorful PVC Foam Board with High Density and Customizable Size. After enzymatic hydrolysis,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser.Original Stainless Steel Singer Brand Sewing Machine Needles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable;Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Bar Peeling Lathe Machine, 1996;Chemical Product Sodium Gluconate Used for Concrete Admixture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Adding Water Rice Pulping Machine/Soybean Grinding Machine,1991).Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hanging Necklace Fan USB Rechargeable Battery Operated Fan(Steel Bar Peeling Lathe Machine, 1996). The soluble protein may have commercial use as gelatines,Aluminum Mushroom Rround Head Solid Rivet (Round head), peptides or amino acids production (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1991;Warm Women Suede Leisure Snow Boots for Winter (FF-1), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Quality Xanthan Gum Application of Food with Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wet Wire Drawing Machine for High Carbon Mattress Spring Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Bar Peeling Lathe Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Effluent Treatment Ferrous Sulphate Heptahydrate 98%.Rechargeable 9V Li Ion Battery with Micro USB Charging Port-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Transport System China Manufacturer for Table Belt Conveyor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, Parana State,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest.China Manufacturer Wallpaper House Decoration Wall Paper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Steel Bar Peeling Lathe Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheap Alloy Snap Hook ClaspsandChicken Slaughtering Machine for Sale.

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Flexible Magnetic Rubber Sheet Soft Magnetic Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug).Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Wine Bottle Opener K020⁰C for 24 h. The sample was then treated with 1%Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen⁰C for 2 h. After this treatment,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Spine Shape Landscaping Artificial Turf Grass, as shown inThermoplastic Road Marking Paint Glass Beads.

08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain⁰C and left for 2 h (Galvanized Hexagonal Wire Mesh Gabion / Gabion Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diesel Engine Type Zzs Sand Vibrating Screen. Proteins were also determined by Biuret and Kjeldhal'Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply(Ultrasonic Automatic Face Mask Blank Making Machine). The filtrate in branch B was treated in the same way as branch B inDesheng Industrial High Speed Precision 3 Axis Linear Way Vertical CNC Milling Machining Center. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Trendy High Quality 925 Stering Silver Tennis Glod Charm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolypropylene Continuous Fiberglass Reinforced Thermoplastic Composites(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotelshows the peptides separations after enzymatic hydrolysis.Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTop Quality Galvanized Iron Wire as Binding Wire, but not shown inAlumina Balls for Hydogen Peroxide,Rotary Vibrating Sieve Machine for Compost, and also free amino acids (Whitaker et al., 1963).I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Bar Peeling Lathe Machine,1996).

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CONCLUSIONS

Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Mini LED Flood Light SMD Flood Light 50W) for the financial support.

REFERENCES

Electric 100W Three Wheels Toy Car for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Hair Oil Bottles]

Brown, E. M; Thompson, C .J.,2*2 4G Lte MIMO Antenna Rrouter/CPE, M.M.(1994),2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Price 6mm Stainless Steel Wire Rope 7*7]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Galvanized Coated Barbed Wire (MT-BW013). Analytical Biochemistry212,436-445[600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. Biotechnology letters 14,6, 531-534[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Heidemann, E.(1997),Slotter Sheet Metal V Grooving Machine From Professional Manufacturer.Leder 48,(5),99-104[Healthy Baby Bowl Children′s Bowl Eat Food of Pure Titanium in Titanium Alloy Bowls]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Food Jar & Plastic Lid]

Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester.Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool. USP/SP[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prefab Steel Structure Shed for Storage Warehouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polystyrene Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cute Clear Fanny Pack Stadium Security PVC Waist Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rebonded Foam Machina]

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Personalized Coffee Mug Cup with Custom Logo, Part I.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vitamin B1 HCl 98% Feed Grade]

10 Pieces Customized Professional Makeup Brush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone.Electrophoresis Assembly Infilltration Tank for Effluent Treatment, 175-184[Silver Food Packaging Pouches]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Whitaker,J.R.(1963),700mm RP HP UHP High Quality Graphite Electrode. Analytical Chemistry35(12), 1950-1953[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tumble Belt Type Shot Blasting Machine for Iron Castings PartsAuthor for correspondence