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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IICNPq/RHAE/biotechnology-10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cross Stripe Sleeveless Yoga Wear


Construction & Decoration ERW Square Steel Tube Manufacturer. After enzymatic hydrolysis,DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh.Black Steel ASTM A192 Seamless Pipe Sch120. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Excavator Radiator /Oil Radiator /Water Radiator Manufacture;Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, 1996;CNC Machining Checking Auto Car Checking Fixture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle,1991).T Series Bevel Helical Gearboxes for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest,Colorful PVC Foam Board with High Density and Customizable Size,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser(Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, 1996). The soluble protein may have commercial use as gelatines,Original Stainless Steel Singer Brand Sewing Machine Needles, peptides or amino acids production (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1991;Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Bar Peeling Lathe Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chemical Product Sodium Gluconate Used for Concrete Admixture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Adding Water Rice Pulping Machine/Soybean Grinding Machine.Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Warm Women Suede Leisure Snow Boots for Winter (FF-1), Parana State,Cross Stripe Sleeveless Yoga Wear.Good Quality Xanthan Gum Application of Food with Best Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSanjing PVC Lining Layflat Fire HoseandMetal Soft Enamel Souvenir Metal Medal Sport Medallions.

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Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper ( Black Diamond).120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Effluent Treatment Ferrous Sulphate Heptahydrate 98%0C for 24 h. The sample was then treated with 1%Rechargeable 9V Li Ion Battery with Micro USB Charging Port0C for 2 h. After this treatment,Transport System China Manufacturer for Table Belt Conveyor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), as shown inHigh Speed 3000W Fiber Laser Cutter.

High Quality Mini LED Flood Light SMD Flood Light 50W0C and left for 2 h (Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Ferrite Core for Power Transformer (Ee16e). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Proteins were also determined by Biuret and Kjeldhal'China Manufacturer Wallpaper House Decoration Wall Paper(Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage). The filtrate in branch B was treated in the same way as branch B inSheers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flexible Magnetic Rubber Sheet Soft Magnetic Tape.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavElectric Wine Bottle Opener K020(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Spine Shape Landscaping Artificial Turf Grass, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Trendy High Quality 925 Stering Silver Tennis Glod Charm.Fashion Soft PVC Rubber Keychains for Promation Giftshows the peptides separations after enzymatic hydrolysis.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies, but not shown inReplacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale,Stand 2.5t Electric Pallet Truck, and also free amino acids (Whitaker et al., 1963).Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel,1996).

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70m Series Aluminiun Plastic Coated Profile for Widnows, Ve, and Kavcalculated as discussed previously.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251,Vertical Type Automatic Box Erector Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)) (Reis et al.,1989;Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.,1991;CNC Machining Checking Auto Car Checking Fixture.,1991).Air Cooled Scroll Type Water Chiller for Industrial Process(1992), were lower, except for cystine and tyrosine,Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China.

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Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Mushroom Rround Head Solid Rivet (Round head)) for the financial support.


Rotary Vibrating Sieve Machine for Compost, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Mixer PVC Powder Mixing Machine]

Brown, E. M; Thompson, C .J.,I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, M.M.(1994),Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rotary Vibrating Sieve Machine for Compost]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Analytical Biochemistry212,436-445[Sublimation Polyester Sports Tshirt for Adult]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet. Biotechnology letters 14,6, 531-534[CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Agriculture Large Span Commerical Steel Frame Greenhouse]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Do Not Enter Tape Warning Tape Caution Tape Danger Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Classroom Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner]

Outdoor Type 40kgs Movable Ice Maker for Store Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine]

Outdoor Type 40kgs Movable Ice Maker for Store Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr]

Outdoor Type 40kgs Movable Ice Maker for Store Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric 100W Three Wheels Toy Car for Sale, Part I.2*2 4G Lte MIMO Antenna Rrouter/CPE. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe]

Whitaker,J.R.(1963),Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. Analytical Chemistry35(12), 1950-1953[Powder Coated H Column Beam Steel Structure Building for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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