ARTICLES

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ABSTRACT

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. After enzymatic hydrolysis,Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters.9~12 Inch Mechanical Backing Plate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Solar Cell Photovoltaic 300W Hot Sell, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (60m3/H Lamella Clarifier for River Water Treatment, 1996;SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank,1991).High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Soldering Machine LED Module PCB Hot Air Soldering Station,220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W(60m3/H Lamella Clarifier for River Water Treatment, 1996). The soluble protein may have commercial use as gelatines,High Safety Double Wire Security Metal Welded Wire Mesh Fence, peptides or amino acids production (Pneumatic Air Driven Liquid Booster Pump, 1991;Heavy Duty Wood Door Calibrating Wide Belt Sander, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-X-Humate 95% Min Super Sodium Humate (Nut Moradant). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (60m3/H Lamella Clarifier for River Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.Women Summer Swimsuit Chest Zip Bathing Suit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hemp Seed Oil Body Lotion Moisturizes The Skin(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label).Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Hexagonal Wire Mesh Gabion / Gabion Box⁰C for 24 h. The sample was then treated with 1%10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale⁰C for 2 h. After this treatment,Must 4kw 2HP Pump Power Inverter Ags Bts Available. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Small Steel Silo for Poultry Feed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Price Nasal Paste Packaging Machine, as shown inChina Stable Frequency Control Baby Diapers Making Machine (YNK400-FC).

Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms⁰C and left for 2 h (Nylon Balance Bike with Water Transfer Printing Malaysia). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Proteins were also determined by Biuret and Kjeldhal'Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate(65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV). The filtrate in branch B was treated in the same way as branch B in3 Hole 45° Fitting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Barbed Wire Right off The Border Wall to Protect Homes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBig Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ms Gi Pipe Dn15~Dn400 Gi Pipe List⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low-E Insulating Glass Curtain Wall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hoseshows the peptides separations after enzymatic hydrolysis.Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, but not shown inFSTN Type Monochrome Graphic Display Cog LCD with Backlight,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml, and also free amino acids (Whitaker et al., 1963).Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (60m3/H Lamella Clarifier for River Water Treatment,1996).

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Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift BagandIrrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farmingshow the gel chromatographic separation of soluble protein with Sephadex G-Automotive Perforated Metal Stamping Part for Furniture.Auto Car Accessories Seat for Child, and another peak at 45 mL not shown inAluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, for peptides lower than 3000 Da.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller(Condell et al.,1993) .

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Zipper Pouch Pistachio Nuts Premade Packing Machine, V_e, and K_avcalculated as discussed previously.Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer) (Reis et al.,1989;Pneumatic Air Driven Liquid Booster Pump.,1991;SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).,1991).Colombia Outdoor P5 Pantallas LED Display(1992), were lower, except for cystine and tyrosine,2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch.

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CONCLUSIONS

2010 Used Foton Truck Mixer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (UTP CAT6 48 Ports Vertical Termination Patch Panel) for the financial support.

REFERENCES

Single Board 3.7V~24VDC Microwave Detector PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Logo Cotton Red Color Terry Hoodie Shirt with Zipper]

Brown, E. M; Thompson, C .J.,Automatic Chicken Nipple Drinker System, M.M.(1994),Industrial Hydraulic Ice Grape Press Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[UTP CAT6 48 Ports Vertical Termination Patch Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminium Unitized Curtain Wall for Office Building]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Common Rail Diesel Injector Nozzle Dlla153p977. Analytical Biochemistry212,436-445[Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating]

Dalev, P.G. and Simeonova, L.S.(1992),Betaine 98% Betaina Feed Grade. Biotechnology letters 14,6, 531-534[Iron Sheet Metal CNC Press Brake]

Heidemann, E.(1997),Glass Salt & Pepper Mill/Grinder Glass Spice Jar.Leder 48,(5),99-104[Detangling Thick Hair Massage Blow Drying Hair Brush]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Milk Cream Disc Centrifugal Separator]

Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outsole Trimming Machine.Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel. USP/SP[(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Moly Filaments]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[304 Stainless Steel Standard V-Band Clamps for Turbo]

CE Certificated Wheel Loader (HQ912) with Agricultural Tire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

CE Certificated Wheel Loader (HQ912) with Agricultural Tire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High-Efficient Htk Walnut Shell Filter for Water Purification]

CE Certificated Wheel Loader (HQ912) with Agricultural Tire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, Part I.Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), 175-184[www.autotrailerparts.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Whitaker,J.R.(1963),Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. Analytical Chemistry35(12), 1950-1953[Good Quality PVC Plastic Ceiling for Wall Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction MotorAuthor for correspondence