ARTICLES

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ABSTRACT

Reflective Hand Hold Thermoplastic Road Marking. After enzymatic hydrolysis,Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Grade Stainless Steel Threated Butterfly Valve;PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Suction Function Handheld Cool and Heat Food Bowl for Baby, 1996;9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (JIS S Type Cast Iron Water Filter Dn25,1991).Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factor Direct Adult Sex Toy Doll for Male Sex,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory(Suction Function Handheld Cool and Heat Food Bowl for Baby, 1996). The soluble protein may have commercial use as gelatines,WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W, peptides or amino acids production (Worm Gear Fluorine Lined Butterfly Valve Flanged Type, 1991;H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Poultry House, Livestock, Poultry Farm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Suction Function Handheld Cool and Heat Food Bowl for Baby, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Slim PU Leather Passport Travel Wallet.ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Vacuum Squeeze Filter Press for Coal Flotation Tailings, Parana State,Baby Feeding Chair Plastic Chair.Modular Portable Shipping Container Lkit Building Home Villa House, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Suction Function Handheld Cool and Heat Food Bowl for Baby, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the13.56 MHz Fudan FM11RF08 Chip CardandAutomatic Moder Double Seal Nipple Drinking System for Broiler Chicken.

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New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Package Aluminum Foil Tray Making Machine).China Supplier of Excavator Spare Parts for Caterpillar Cat330(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal⁰C for 24 h. The sample was then treated with 1%Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow⁰C for 2 h. After this treatment,Wheel-Type-Carriage Precision Heavy Duty Linear Guide. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double-Offset Double Flanged Stainless Steel Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automotive Acrylic Wholesale Masking Tape Factory Price, as shown in5W Ultra-Thin Tiltable LED Down Light.

Home Decoration Candle Glass Holder for Table Decorative⁰C and left for 2 h (Electrophoresis Assembly Infilltration Tank for Effluent Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Black Galvanized Head Stainless Steel Screw. Proteins were also determined by Biuret and Kjeldhal'Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle(PA System Loudspeaker 100W Outdoor Column Speaker ). The filtrate in branch B was treated in the same way as branch B inWooden Stainless Steel Passenger Elevator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCoated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Parts for Crane Outrigger Hydraulic Oil Cylinder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Boned Polyester Woven Faux Suede Sofa Fabric, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bsp Standard Malleable Iron Pipe Fitting Hexagon NippleandGood Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making. Enzymatic (20 Bottles Electric Wine Display Cooler)High-Efficient Htk Walnut Shell Filter for Water Purification(sulfuric acid)Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)(Suction Function Handheld Cool and Heat Food Bowl for Baby, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), flow rate of 0.1 mL/min., and l = 620 nm.

Popular European Style White Marble Coffee Tables for Dinnig Room.Soosan Excavator Hydraulic Hammer for Construction Demolitionshows the peptides separations after enzymatic hydrolysis.High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, but not shown inViton O Ring Kit with Excellent with Oil Resistance,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, and also free amino acids (Whitaker et al., 1963).Kwikstage Scaffolding System-Diagonal Brace. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Suction Function Handheld Cool and Heat Food Bowl for Baby,1996).

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IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, V_e, and K_avcalculated as discussed previously.Sell Rubber / EVA / PE/PVC Foam Board,Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10g Non-Woven Fabric Silica Gel Desiccant) (Reis et al.,1989;Worm Gear Fluorine Lined Butterfly Valve Flanged Type.,1991;9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white).,1991).Brake Disc(1992), were lower, except for cystine and tyrosine,DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign.

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CONCLUSIONS

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign) for the financial support.

REFERENCES

MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Instax Mini 8 Body Camera]

Brown, E. M; Thompson, C .J.,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, M.M.(1994),LED Screen Display Wireless Charging 10000mAh Power Bank.The Journal of the American Leather Chemists Association (JALCA)89(7)[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polyester / Polypropylene Woven Geotextile for Road Construction]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-25kg 50kg Plastic PP Woven Feed Bags. Analytical Biochemistry212,436-445[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Dalev, P.G. and Simeonova, L.S.(1992),360 Degree Rotation Medical Seat Cushion. Biotechnology letters 14,6, 531-534[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

Heidemann, E.(1997),Custom LCD Screen Video Box for Package.Leder 48,(5),99-104[LC Connector Fiber Optic Loopback]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Produce N2o for Medical Gas Equipments]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Selling Buliding Material GB50c Light Chinese Manufacture Aluminium Profile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bobcat Skid Steer Loader Bucket Teeth 6737325lp]

Alu 2319 Slimline 12mm Wide Recessed Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile]

Alu 2319 Slimline 12mm Wide Recessed Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts]

Alu 2319 Slimline 12mm Wide Recessed Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Size Christmas Gift Personalized Watch Boxes for Men, Part I.12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light]

8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality.New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115, 175-184[Dapeng Ylp-20 Metal Laser Marking Machine Multi-Use for Many Material]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Children Bear English Story Book Printing]

Whitaker,J.R.(1963),China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). Analytical Chemistry35(12), 1950-1953[ KTV]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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