Services on Demand
Journal
Article
Indicators
Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)
High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry
Related links
Economical Residential Home Automatic Aluminum Rolling Shutter Door
Share
China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Viola Bow
ARTICLES
Clear PVC Sheet with Coating for Card
Astm 80Edivaldo E.GarciaI; Cláudia C.M.Classic Black and White Plaid Sling Bag for WomenI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiElectrical 4W AC Synchronous Gear Motor for Engraving Machine
IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor
ABSTRACT
Free Sample E Liquid/E-Juice for Nicotine Vaping. After enzymatic hydrolysis,Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft.100% Acrylic Knitted Sports Woven Scarf. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Prefab Steel Structure Shed for Storage Warehouse;Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Gen 2+ or 3 Day/Night Vision Scope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1996;3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1650mm*80mm Stable Power Laser Tube,1991).FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Lead Ingot 99.994 Reasonable Price,Micro-Combustion Tube, Test Tube (HK-224),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen(Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1996). The soluble protein may have commercial use as gelatines,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, peptides or amino acids production (3W LED Explosion Proof Emergency Exit Light, 1991;Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stationery PP Clipboard With 7 Inch Scale Ruler., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10PVC Cross Joint Pipe Fittings Equal Pipe Cross.3PC Stainless Steel Flanged End Ball Valve 3 Inches 150-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ysdcnc Manual Sheet Metal Plate Bending Roller Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
Betaine Anhydrous 98% Food Grade Good Quality Competitive PriceAlcalase2.5 L DX,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Bearing Stand Type Cam Follower); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hot Sale Electric LED light Aroma Diffuser(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, Parana State,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale High Quality Metal Car Mount Holder Phone HolderandHome Decoration Kids Toy Promotion Eyeglass Plastic Mould.
Adjustable Aluminum Solar Mounting System
15000lbs 4X4 Electric Winch for 4WD
Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Stand 2.5t Electric Pallet Truck).7PCS RGBW LED Plastic PAR Light Wall Washer(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm0C for 24 h. The sample was then treated with 1%Kindergarten Playground Preschool Kids Plastic Slide and Swing0C for 2 h. After this treatment,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Railway Freight Wagon Spare Parts Casting and Forging for Bogie, as shown inCross Stripe Sleeveless Yoga Wear.
Rainsky Ceiling Mounted Stainless Steel Shower Heads0C and left for 2 h (OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Sizes Acrylic Shoes Storage Display Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,High Quality Casting Cover Aluminum Die Casting Products. Proteins were also determined by Biuret and Kjeldhal'Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags(Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon). The filtrate in branch B was treated in the same way as branch B in2019 Woman Handbag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavExcellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut(HPLC)
The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/304 316 Stainless Steel Grooved Cross for Water, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Precision Engineering Parts Bearing Support Unit for Ball ScrewandOutdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. Enzymatic (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home)20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(sulfuric acid)Hot Sale Laser Projector RGB Laser Light for Stage(Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, flow rate of 0.1 mL/min., and l = 620 nm.
Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.Discussion and Interpretation Systemshows the peptides separations after enzymatic hydrolysis.Acm Dewatering Twin Screw Extruder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUV Laser Marking Machine (with Air Cooling System), but not shown in12V Electric Portable Outdoor Water Fruit Car Fridge with Ce,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), and also free amino acids (Whitaker et al., 1963).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved,1996).
Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall
Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace
100% Polyester Gabardine Shirting Fabric with Transfer PrintingTreeandCopper Laminated Steel Strip T2/C11000show the gel chromatographic separation of soluble protein with Sephadex G-Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.Good Quality Good Price China Plastic Injection Molding Machine, and another peak at 45 mL not shown in Rubber Wrist Bands, for peptides lower than 3000 Da.Pet Hair Remover Glove for Dog, Grooming Gloves Brush(Condell et al.,1993) .
Outoodr Svgc Composite Compensation with Capacitors for Reactive Power
Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, Ve, and Kavcalculated as discussed previously.Alumina Balls for Hydogen Peroxide,Anti-High Pressure Insulated Pliers with PVC Dipped Handles. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Water Filter Polyester Non-Woven Fabric) (Reis et al.,1989;3W LED Explosion Proof Emergency Exit Light.,1991;3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA.,1991).Hot Selling Electronical Blue Pet Plastic Release Film Price(1992), were lower, except for cystine and tyrosine,DIN127 Stainless Steel Spring Lock Washer.
Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price
Colorful PVC Foam Board with High Density and Customizable Size
CONCLUSIONS
29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bearing Stand Type Cam Follower) for the financial support.
REFERENCES
New Condition Hot Sale Automatic Animal Feed Cooler Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Quarter Round Corner Tile Trim]
Brown, E. M; Thompson, C .J.,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, M.M.(1994),Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144).The Journal of the American Leather Chemists Association (JALCA)89(7)[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[X Shaped Extruded Aluminum Window Screen Frames]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garlic Red Mesh Bag. Analytical Biochemistry212,436-445[Personalized Coffee Mug Cup with Custom Logo]
Dalev, P.G. and Simeonova, L.S.(1992),Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. Biotechnology letters 14,6, 531-534[OEM Metal Stamping Stainless Steel Parts with CNC Machining]
Heidemann, E.(1997),Ground Finish Molybdenum Tzm Rod.Leder 48,(5),99-104[Carbide Cnc Router Bit Wholesaler Quotes & PriceList]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Driving System for Hybrid Stepper Motor Dm860A]
Classic Black and White Plaid Sling Bag for Women, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Transparent/Clear BOPP Jumbo Roll.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. USP/SP[Small Backhoe Loader with Air Conditioning]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chenille Microfiber Mop Head: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4 Drawer Steel Office Vertical Filing Cabinet]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2oz 3oz 4oz 6oz 8oz 12oz 16oz Bid Wide Mouth Straight Kitchen Empty Tea Food Bamboo Lid Storage Glass Jars]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Insecticidal Lamp with Solar Energy Frequent Vibration]
Custom Waterproof Pizza Sign Large Advertising Signboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Shrink Sleeve Label for Drinking Water Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]
Custom Waterproof Pizza Sign Large Advertising Signboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]
Custom Waterproof Pizza Sign Large Advertising Signboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, Part I.Galvanized Square Wire Mesh, Metal Window Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sequins Butterfly Handmade Beads Embroidery Patch]
Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter.OEM Customized Stainless Steel Parts by Deep Drawing, 175-184[Znse Zinc Selenide Laser IR Lens]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]
Whitaker,J.R.(1963),Children Bear English Story Book Printing. Analytical Chemistry35(12), 1950-1953[50mm Magnetic River Water Sewage Flow Meter for Acid]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)Author for correspondence