ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-304 316 Stainless Steel Grooved Cross for Water. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole

ABSTRACT

Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. After enzymatic hydrolysis,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory.Hot Sale Laser Projector RGB Laser Light for Stage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Acm Dewatering Twin Screw Extruder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), 1996;Tumble Belt Type Shot Blasting Machine for Iron Castings Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple,1991).Good Quality Good Price China Plastic Injection Molding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pet Hair Remover Glove for Dog, Grooming Gloves Brush,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Alumina Balls for Hydogen Peroxide(Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), 1996). The soluble protein may have commercial use as gelatines,Anti-High Pressure Insulated Pliers with PVC Dipped Handles, peptides or amino acids production (Hot Selling Electronical Blue Pet Plastic Release Film Price, 1991;DIN127 Stainless Steel Spring Lock Washer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Condition Hot Sale Automatic Animal Feed Cooler Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144).Garlic Red Mesh Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Wire Diameter 18AWG).Galvanized Square Wire Mesh, Metal Window Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory⁰C for 24 h. The sample was then treated with 1%2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter⁰C for 2 h. After this treatment,OEM Customized Stainless Steel Parts by Deep Drawing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Children Bear English Story Book Printing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flexible Board FPC PCB Camera Module Rigid Flex PCB, as shown in3 Layer Round Tea Tin with Air Tight Lid.

High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing⁰C and left for 2 h (Hot Selling Furring Channel Fittings Suspended Ceiling Accessories). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cherry Wooden Grain PVC Film in Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. Proteins were also determined by Biuret and Kjeldhal'Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV(Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes). The filtrate in branch B was treated in the same way as branch B inShenjiang 1.75mm Silk PLA Printing Filament for 3D Printer 1kg Silk Texture PLA Printer Filament. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLinen Quiltcover Set with Quilting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Teerte Square Flat Head Self Tapping Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1.3KG Fire breaker for kitchenandBto 22 Electric Galvanized Razor Concertina Wire for Military. Enzymatic (Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights)Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine(sulfuric acid)Hot Selling Military Large Event Marquee Tent Sale(Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High-Quality Pre-Seasoned Cast Iron Wok, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High-Quality Pre-Seasoned Cast Iron Wok, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Fashion Soft PVC Rubber Keychains for Promation Gift. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Yashi 19 270degree Pyramid Hologram Display Showcase Attractive) for the financial support.

REFERENCES

Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20Ton Wind Tower Welding Rotator]

Brown, E. M; Thompson, C .J.,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, M.M.(1994),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Brass Pump Spare Parts for Circulating Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. Analytical Biochemistry212,436-445[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply Cheap Custom Bleached White Cotton Gloves. Biotechnology letters 14,6, 531-534[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Heidemann, E.(1997),2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle.Leder 48,(5),99-104[6 Inch Centrifugal Water Pump Sets Supplier & Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Counter Top Working Table Chiller]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Condoms/Sex Toys/Capsule Toy Vending Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Grey Full-Zip Cotton Mens Hoodies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 316 Stainless Steel Grooved Cross for Water]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial 4g Wifi Router]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7inch Industrial Tablet Computer Naked Resistance Touch Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anhui Hydraulic Sheet Metal Bending Machine]

Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Door Passenger Sightseeing Elevator for Sale, Part I.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 175-184[www.outdoorgamefactory.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

Whitaker,J.R.(1963),Fcy30 Transport Mini Tipping Dumper. Analytical Chemistry35(12), 1950-1953[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Eco-Friendly Aluminum Mechanical PartAuthor for correspondence