ARTICLES

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ABSTRACT

Manual Sealing Machine for Instant Noodles Paper Soup Bowls. After enzymatic hydrolysis,Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck.Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion;Corrugated Steel Sheet Corrugated Steel Roofing Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Factory Queen Mattress with Visco Memory Foam and Latex, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1996;Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Hot Forging Agricultural Spare Parts,1991).16mm 12 Strand UHMWPE Synthetic Fiber Rope. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems,Vertical Type Caseformer Machine WJ-LKX-15,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine(High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1996). The soluble protein may have commercial use as gelatines,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, peptides or amino acids production (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, 1991;Agriculture Large Span Commerical Steel Frame Greenhouse, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine.Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chinese Jbcz Handy Operation Threading Machine and Coupler(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, Parana State,Small Manufacturing Tht/ Ai Double Wave Soldering Machine.Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian HairandAluminum Low Profile Adjustable Bracket.

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Bottom Pushing for Fire Extinguisher Hot Spinning Machine⁰C and left for 2 h (Pen Type pH Meter pH-031&0.00-14.00pH Tester). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fish Scales Texture Fashion Leather for Shoe (S312100GH). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diamond Segments 2000mm Diameter Granite Cutting Blade. Proteins were also determined by Biuret and Kjeldhal'Produce N2o for Medical Gas Equipments(Stamping Metal Parts Manufacturer Sheet Parts Fabrication). The filtrate in branch B was treated in the same way as branch B inPrivate Label High Quality Fashion Brand Name Women′s Pants. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Selling Furring Channel Fittings Suspended Ceiling Accessories.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%90m3 Per Hour Mobile Concrete Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.All Weather Rattan Handwoven Swing Outdoor Home⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pond Self Priming Booster suction  Water Pump with Pressure Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door HingeandModern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Enzymatic (for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile)100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree(sulfuric acid)School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlightshows the peptides separations after enzymatic hydrolysis.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Efficiency Solar Cell Photovoltaic 300W Hot Sell, but not shown inTc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China,125kHz RFID Hotel Key Card with T5577 Chip, and also free amino acids (Whitaker et al., 1963).USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing,1996).

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10mm Red Green Yellow Warning China Supplier Signal Lamp, V_e, and K_avcalculated as discussed previously.Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flexible Magnetic Rubber Sheet Soft Magnetic Tape) (Reis et al.,1989;0.1mm Tungsten Carbide 2f Single Flute Micro End Mill.,1991;Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.,1991).Phillip Countersunk Head Screw Hot DIP Galvanized(1992), were lower, except for cystine and tyrosine,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.

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CONCLUSIONS

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Price Printing Doy Pack Bag) for the financial support.

REFERENCES

Heat Shrink Automatic Flow Wrapping Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pu Injection Moulding Machine]

Brown, E. M; Thompson, C .J.,Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, M.M.(1994),Ductile Iron Standard Brackets Pipe Hanger.The Journal of the American Leather Chemists Association (JALCA)89(7)[100%Polyester Satin Fabric for Blouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. Analytical Biochemistry212,436-445[Lantern for Garden Decoration]

Dalev, P.G. and Simeonova, L.S.(1992),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. Biotechnology letters 14,6, 531-534[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

Heidemann, E.(1997),5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building.Leder 48,(5),99-104[Vacuum Water Pump Air Cylinder Pressure Sensor (JC627-30)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi-Function Small Tree Digging Shovel/Tree Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1 Ton Chain Pulley Block with Geared Trolley]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Spooling Pet Polyester Mylar Film for Cable Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5 Star Hotel Standard Spring Foam Mattress with Natural Latex]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Micro-Combustion Tube]

Frame Type Rubber Hydraulic Press Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kitchen Metal Dish Storage Rack (JP-SC984C)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chemical Product Sodium Gluconate Used for Concrete Admixture]

Frame Type Rubber Hydraulic Press Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket]

Frame Type Rubber Hydraulic Press Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, Part I.AVR Se350 Automatic Voltage Regulator for Marathon Alternator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit]

Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.High Strength Rigid PVC Plastic Foam Board for FRP, 175-184[Juicer Meat Grinder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer Industrial Aluminum Floor Bar Grating]

Whitaker,J.R.(1963),Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. Analytical Chemistry35(12), 1950-1953[Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal InductorAuthor for correspondence