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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Industry Pet Recycling Systems, Industry Pet Recycling Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sublimation Polyester Sports Tshirt for Adult

ABSTRACT

Heavy Duty Marquee Advertising Pop up Tent. After enzymatic hydrolysis,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer.Fire Extinguisher UV Curing Machine Cylinder Drying Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Valve Bag Dry Mortar Bagging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 500m 100lb Super Strong Braided Fishing Line, 1996;20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light,1991).Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint,Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending(High Quality 500m 100lb Super Strong Braided Fishing Line, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, peptides or amino acids production (Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, 1991;Factory Directly Selling Engine Standard Spark Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Logo Silicone Wristband, Rubber Wrist Bands., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom All Kinds of Acrylic Shoe Display Racks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 500m 100lb Super Strong Braided Fishing Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.Medical Supply U40 Disposable Insulin Syringe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board).Cow Dung Chicken Manure Organic Fertilizer Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%marble Makeup Brush Set for Travel in 20PCS⁰C for 24 h. The sample was then treated with 1%Swimming Pool Water Disinfect Ozone Generator⁰C for 2 h. After this treatment,20kv 50kVA High Voltage Dry Type Power Supply Transformer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, as shown inHot Sale Spraying Drying Equipment.

4 Styles Blue Glass Crystal Butt Beads Spiral Plug⁰C and left for 2 h (Heating Refrigerated Low Temperature Cooling Circulator Water Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Activated Carbon Water Filter Industry Water Treatment. Proteins were also determined by Biuret and Kjeldhal'2016 New Generation Sausage Making Machine(Custom Design Plush Panda Animal Slippers Comfort Foam Support by). The filtrate in branch B was treated in the same way as branch B inCustom Design Recyclable Paper Corrugated Advertising Pop Cardboard Display Stand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Magnetic Mixer Mixing Tank with Bottom Agitator (MTK).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lab Equipment portable Fume Hood, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Precision Tungsten Carbide Machine Customized Cutting Tool) for the financial support.

REFERENCES

Nylon Cable Glands with CE, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bags Women Handbags Tote Supplier & Factory]

Brown, E. M; Thompson, C .J.,X Shaped Extruded Aluminum Window Screen Frames, M.M.(1994),Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. Analytical Biochemistry212,436-445[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Dalev, P.G. and Simeonova, L.S.(1992),4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. Biotechnology letters 14,6, 531-534[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]

Water Filter Polyester Non-Woven Fabric, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD.Low Power Consumption Laser Foam Vertical Die Cutting Machine. USP/SP[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.asanotech.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Supply 100% Natural Epimedium Extract for Male Health]

Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Acrylic Beer Display Racks with LED]

Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211), Part I.Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[T6 Outdoor Waterproof Tactical Flashlight]

High Quality Crusher for Used Plastic Recycling Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit.SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, 175-184[Blue Hdpe Water Supply Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Whitaker,J.R.(1963),Wear Resitant Rope End Cap Plastic Flange Cover. Analytical Chemistry35(12), 1950-1953[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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