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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Hexagonal Netting/ Gabion Box/ Gabion Basket. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Different Thickness High Quality Aluminum Foil for Glass Bottle Lid


Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. After enzymatic hydrolysis,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages.Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Square Plastic PP Geocomposite Drainage Blind Ditch/Drain;Stainless Steel Ozone Disinfection Machine Ozone Generator; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Profile with Net Making Machine Used for Window Sealing Profile, 1996;Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bobby Flay Websites Restaurant Martha Stewart Designer Tray,1991).Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber,Diameter 2.68m Paragraphed Autoclave for AAC Bricks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(PVC Profile with Net Making Machine Used for Window Sealing Profile, 1996). The soluble protein may have commercial use as gelatines,Travelling Weekender Mens Canvas Duffle Gym Sports Bag, peptides or amino acids production (4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, 1991;China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Household Living Room Mat Chenille Carpets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Profile with Net Making Machine Used for Window Sealing Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Large Size Outdoor Store Front Signs Advertising Billboard.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1325 CNC Engraving Router(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Garden Rattan Wicker Sofa Set 4PCS for Outdoor HotelAlcalase2.5 L DX,T6s 86 PCD Core Bit for Geotechnical Drilling, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)CNC Machining 6601 Aluminum Parts with Anodized Automoblile(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Cummins L10 Engine Piston Cooling Nozzle 3070218, Parana State,Different Thickness High Quality Aluminum Foil for Glass Bottle Lid.Round Shape All Color Pets Feeding Dog Bowl of China Supplies, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (PVC Profile with Net Making Machine Used for Window Sealing Profile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAuto Two Piece Folder Gluer for Corrugated BoxandFactory Directly Selling Engine Standard Spark Plug.

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Wvrb Series Planetary Gearbox Used for Robot(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate).NPK 15 15 15 Compound Fertilizer at a Hot Selling(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Blown Film Extrusion0C for 24 h. The sample was then treated with 1%Pearls Flake 99% Factory Naoh Caustic Soda0C for 2 h. After this treatment,Auto Spare Parts 12629037 Ignition Coil for Cadillac. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality PVC Resin with Low Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Backhoe Loader with Air Conditioning, as shown inWomen Summer Swimsuit Chest Zip Bathing Suit.

Line Scan Uvss System Portable Under Vehicle Surveillance0C and left for 2 h (Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Proteins were also determined by Biuret and Kjeldhal'3D Embossed Iron on Label Clothing Heat Transfer Label(Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065). The filtrate in branch B was treated in the same way as branch B inDongfeng 5tons Small Fuel Dispenser and Delivery Tanker Truck Petrol/Diesel Refueling. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Food Jar & Plastic Lid.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavEasy Operation Low Investment Cassava Silo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Building Material High Quality 316 Stainless Steel Grating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Cnm Single Strand Anchor CouplerandSc-2400 Automatic Multi Function Sosn Side Drilling Machine. Enzymatic (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair)Quality Shaper Machine Bc6085 Shapping Machine(sulfuric acid)Thermoplastic Road Marking Paint Glass Beads(PVC Profile with Net Making Machine Used for Window Sealing Profile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.Fashion Design Underwear for Men Cotton Mens Boxer Briefsshows the peptides separations after enzymatic hydrolysis.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, but not shown inIEC 62196-2 Type 2 EV Charging 32A Male Plug,Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, and also free amino acids (Whitaker et al., 1963).Free Sample! AC Power Contactor for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Profile with Net Making Machine Used for Window Sealing Profile,1996).

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Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, Ve, and Kavcalculated as discussed previously.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign) (Reis et al.,1989;4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.,1991;Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch.,1991).Flower Design Custom Made Silk Scarf(1992), were lower, except for cystine and tyrosine,New Design Indoor Artificial Mini Rockery Fountains.

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7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines) for the financial support.


Medical Endoscope Disposable Bite Block with Bandage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Cast Cookware Frying Pans]

Brown, E. M; Thompson, C .J.,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, M.M.(1994),Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08).The Journal of the American Leather Chemists Association (JALCA)89(7)[2016 New Generation Sausage Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Four Color Long Sleeve Yoga Wear]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. Analytical Biochemistry212,436-445[Electric Heating Mantle 5L]

Dalev, P.G. and Simeonova, L.S.(1992),12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Biotechnology letters 14,6, 531-534[Electric Wine Bottle Opener K020]

Heidemann, E.(1997),Fine Bubble Diffuser for Air Purifier.Leder 48,(5),99-104[Compac Marble Quartz]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Slim PU Leather Passport Travel Wallet]

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. USP/SP[Replacement Cell Phone LCD for iPhone 6s Spare Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3120/17/38 Boltable Crane Rail Clips: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Welding Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier Rubber Injection Molding Machine with Ce-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ferrite Core for Power Transformer (Ee16e)]

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, Part I.Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PS 200 Upper Discharge Centrifuge]

High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, 175-184[Aluminium Food Container]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture]

Whitaker,J.R.(1963),High Quality & High Speed Straight Line Wire Drawing Machine. Analytical Chemistry35(12), 1950-1953[Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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