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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Hot Sale Instax Mini Camera Korea Style Photo Album. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Low Profile Adjustable Bracket


Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. After enzymatic hydrolysis,High Quality Acrylic Beer Display Racks with LED.Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Baby Feeding Chair Plastic Chair for Feeding Hebei China;Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Plastic Injection Molding for Auto Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Type Twin Screw Extruder Barrel Manufacturer, 1996;ASA-UPVC Anti-Corrosive Composite Roof Tile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System,1991).Sexy Mesh Transparent Female Running Tank Top with Hood Custom. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Color Cardstock Color Paper Manufacturer,Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Home and Office PVC Vinyl Flooring Conductive Plank Flooring(Round Type Twin Screw Extruder Barrel Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Precision Engineering Parts Bearing Support Unit for Ball Screw, peptides or amino acids production (LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, 1991;165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silicone Cake Mold Sinle Cupcake DIY., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Type Twin Screw Extruder Barrel Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Cross Stripe Sleeveless Yoga Wear.Construction & Decoration ERW Square Steel Tube Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System).T Series Bevel Helical Gearboxes for Sale(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest0C for 24 h. The sample was then treated with 1%Colorful PVC Foam Board with High Density and Customizable Size0C for 2 h. After this treatment,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Original Stainless Steel Singer Brand Sewing Machine Needles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, as shown inHome Use Standing Modern Floor Lamp (KAF6108).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavLm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hanging Necklace Fan USB Rechargeable Battery Operated Fan0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Mushroom Rround Head Solid Rivet (Round head), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


160 Short Pitch Precision Roller Chain High Tensile Strength Roller ChainandWarm Women Suede Leisure Snow Boots for Winter (FF-1). Enzymatic (Good Quality Xanthan Gum Application of Food with Best Price)Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(sulfuric acid)120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome(Round Type Twin Screw Extruder Barrel Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Effluent Treatment Ferrous Sulphate Heptahydrate 98%, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Effluent Treatment Ferrous Sulphate Heptahydrate 98%, flow rate of 0.1 mL/min., and l = 620 nm.

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China Manufacturer Wallpaper House Decoration Wall Paper, Ve, and Kavcalculated as discussed previously.Flexible Magnetic Rubber Sheet Soft Magnetic Tape,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine) (Reis et al.,1989;LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection.,1991;ASA-UPVC Anti-Corrosive Composite Roof Tile.,1991).Electric Wine Bottle Opener K020(1992), were lower, except for cystine and tyrosine,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.

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China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine) for the financial support.


China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[16mm Pp Bio Ball Media]

Brown, E. M; Thompson, C .J.,Spine Shape Landscaping Artificial Turf Grass, M.M.(1994),08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Magic Tape Hook and Loop Fasteners]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. Analytical Biochemistry212,436-445[11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best 6-Axis Electric Screwdriver Machine Robot]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Power Consumption Laser Foam Vertical Die Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Hamburger Speaker for MP3 (XH-PS-007)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Six Stations Pen Press Machine]

Stand 2.5t Electric Pallet Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning]

Stand 2.5t Electric Pallet Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars]

Stand 2.5t Electric Pallet Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, Part I.Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter]

Men Fashion Plaid Design Polyester Ties (WH24), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),70m Series Aluminiun Plastic Coated Profile for Widnows.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 175-184[Triple Action Chlorine 90% Tablets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring]

Whitaker,J.R.(1963),Vertical Type Automatic Box Erector Machine. Analytical Chemistry35(12), 1950-1953[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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