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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. After enzymatic hydrolysis,Supermarket Plastic T-Wire Basket Swing up Price Label Holder.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7;Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Degradable PLA Plastic Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 1996;20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet,1991).2D LED Rope Lighting Motif Lights for Christmas Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls,10ml Dropper Bottle for Skin Care Packing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom High Precision Micro Machining Service Brass Micromachining Parts(Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 1996). The soluble protein may have commercial use as gelatines,7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag, peptides or amino acids production (Replacement Cell Phone LCD for iPhone 6s Spare Parts, 1991;China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Explosion Proof Fog Cannon for Coal Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Silicone Large Non-Stick BPA Free Baking Mat Sheet.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-19mic Black Cast PE Stretch Film for Pallet Wrapping. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens.Custom Design Plush Panda Animal Slippers Comfort Foam Support by-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best 6-Axis Electric Screwdriver Machine Robot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Automatic L Bar Sealer Shrink Tunnel Machine, Parana State,OEM Metal Stamping Stainless Steel Parts with CNC Machining.Full Automatic Carbonated Water Filling Full Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand WomenandTonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine.

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2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Gi/PPGI Roofing Sheet in China).Superior Watertightness JS Polymer Cement Waterproof Coating for Basement(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PS 200 Upper Discharge Centrifuge0C for 24 h. The sample was then treated with 1%3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew0C for 2 h. After this treatment,Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrosurgical Generator High Frequency Electricity Kinfe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Viton O Ring Kit with Excellent with Oil Resistance, as shown in Cheap.

One Step HBV 5 in 1 Rapid Test0C and left for 2 h (China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,2019 Summer Fresh Landscaping Turf Lawn. Proteins were also determined by Biuret and Kjeldhal'Pipe Sheet Laser Cutter with Tube Cutting(2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch). The filtrate in branch B was treated in the same way as branch B inAng Gu Kueh Makingmachine Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSupply Christmas Lace Gold Silver Offray Silk Ribbon(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Toast Stamp Loaf Bread Tin Pan (ZMA)0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Q32 Series Tumble Belt/Crawler Shot Blasting MachineandWiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse. Enzymatic (Baking Soda 99.8% Min Sodium Bicarbonate)BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized(sulfuric acid)4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine(Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, flow rate of 0.1 mL/min., and l = 620 nm.

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Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black Materbatch Suppliers for Sale]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir. Analytical Biochemistry212,436-445[Plastic Food Box Container Covers by Injection Mould Mold Tool]

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Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Large Ss Stamping/ Bending Sheet Metal Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[360 Degree Rotation Medical Seat Cushion]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HDPE Bottle Auto-Deflashing Blow Molding Machine]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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