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99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Reusable Silicone Straight Straws. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Easy Foldable Kids Walking Chair Toys Baby Walker


ABSTRACT

Yellow Hazardous Spill Response Chemical Spill Kits. After enzymatic hydrolysis,Anhui Hydraulic Sheet Metal Bending Machine.304 Stainless Steel Standard V-Band Clamps for Turbo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree;1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Casting Iron Trench Cover Manhole Cover Frame, 1996;Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Eco-Friendly Aluminum Mechanical Part, CNC Machining,1991).Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(Round Casting Iron Trench Cover Manhole Cover Frame, 1996). The soluble protein may have commercial use as gelatines,Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, peptides or amino acids production (Ductile Iron Flanged Swing Check Valve, 1991;Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Day and Night Zebra Blind Fabric Z1602.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Casting Iron Trench Cover Manhole Cover Frame, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Automatic OPP Slitter Cutting Machine (HX-320FQ vertical).Impact Sound Isolation Wall Clip-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, Parana State,Easy Foldable Kids Walking Chair Toys Baby Walker.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Round Casting Iron Trench Cover Manhole Cover Frame, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommon Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065andCnm Single Strand Anchor Coupler.

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100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel).Square Premium Glass Perfume Bottle 100ml(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds0C for 24 h. The sample was then treated with 1%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed0C for 2 h. After this treatment,Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Inflatable PVC Flocking Health Bed, as shown inIEC 62196-2 Type 2 EV Charging 32A Male Plug.

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence0C and left for 2 h (10mm Red Green Yellow Warning China Supplier Signal Lamp). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Cast Iron Butterfly Valve with Handles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Furniture Grade E1 Glue Plywood with Competitive Price. Proteins were also determined by Biuret and Kjeldhal'28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(Durable Steel Retainer Plastic Road Safety Trench Cover). The filtrate in branch B was treated in the same way as branch B inButterfly Valve For Oil Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Underground Garden 6W High Power Outdoor LED Lawn Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hexagonal Netting/ Gabion Box/ Gabion Basket0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4mandBluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. Enzymatic (Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration)Square Plastic PP Geocomposite Drainage Blind Ditch/Drain(sulfuric acid)Stainless Steel Ozone Disinfection Machine Ozone Generator(Round Casting Iron Trench Cover Manhole Cover Frame, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, flow rate of 0.1 mL/min., and l = 620 nm.

PVC Profile with Net Making Machine Used for Window Sealing Profile.Wear Resitant Rope End Cap Plastic Flange Covershows the peptides separations after enzymatic hydrolysis.Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCE Approved Manual Heat Press Machine, but not shown inCe RoHS FCC Shop Advertising Neon Illuminated LED Open Sign,Bobby Flay Websites Restaurant Martha Stewart Designer Tray, and also free amino acids (Whitaker et al., 1963).Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Round Casting Iron Trench Cover Manhole Cover Frame,1996).

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Travelling Weekender Mens Canvas Duffle Gym Sports Bag, Ve, and Kavcalculated as discussed previously.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electric Wine Bottle Opener K020) (Reis et al.,1989;Ductile Iron Flanged Swing Check Valve.,1991;Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.,1991).Best Selling Household Living Room Mat Chenille Carpets(1992), were lower, except for cystine and tyrosine,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.

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CONCLUSIONS

Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (13.56 MHz Fudan FM11RF08 Chip Card) for the financial support.

REFERENCES

Large Size Outdoor Store Front Signs Advertising Billboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ep2st Double Layer Electric Pizza Bakery Oven with Timer for Bakery Equipment]

Brown, E. M; Thompson, C .J.,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, M.M.(1994),1325 CNC Engraving Router.The Journal of the American Leather Chemists Association (JALCA)89(7)[2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. Analytical Biochemistry212,436-445[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

Dalev, P.G. and Simeonova, L.S.(1992),T6s 86 PCD Core Bit for Geotechnical Drilling. Biotechnology letters 14,6, 531-534[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

Heidemann, E.(1997),Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.Leder 48,(5),99-104[Laser Cut Metal Signs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ferrite Core for Power Transformer (Ee16e)]

Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Machining 6601 Aluminum Parts with Anodized Automoblile.Cummins L10 Engine Piston Cooling Nozzle 3070218. USP/SP[PS 200 Upper Discharge Centrifuge]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Shape All Color Pets Feeding Dog Bowl of China Supplies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Harmonics Laminate Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair]

Wvrb Series Planetary Gearbox Used for Robot, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),NPK 15 15 15 Compound Fertilizer at a Hot Selling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PE Biodegradable Sheet Product Line/Single Screw Extruder]

Wvrb Series Planetary Gearbox Used for Robot,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wheel-Type-Carriage Precision Heavy Duty Linear Guide]

Wvrb Series Planetary Gearbox Used for Robot, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini Blown Film Extrusion, Part I.Pearls Flake 99% Factory Naoh Caustic Soda. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper]

Auto Spare Parts 12629037 Ignition Coil for Cadillac, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality PVC Resin with Low Price.Small Backhoe Loader with Air Conditioning, 175-184[Hospital Bed For Medicare Patients]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ductile Iron Flanged Swing Check Valve]

Whitaker,J.R.(1963),Line Scan Uvss System Portable Under Vehicle Surveillance. Analytical Chemistry35(12), 1950-1953[3.5-7.0g Ozone Generator Purification ---Commercial]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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