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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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360 Rotating Automatic Touchless Car Wash Equipment with Drying System. After enzymatic hydrolysis,48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump;Sell Rubber / EVA / PE/PVC Foam Board; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Brake Disc, 1996;DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat,1991).MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder,LED Screen Display Wireless Charging 10000mAh Power Bank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,25kg 50kg Plastic PP Woven Feed Bags(Brake Disc, 1996). The soluble protein may have commercial use as gelatines,360 Degree Rotation Medical Seat Cushion, peptides or amino acids production (Custom LCD Screen Video Box for Package, 1991;Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Alu 2319 Slimline 12mm Wide Recessed Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Brake Disc, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo.Custom Size Christmas Gift Personalized Watch Boxes for Men-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


8p8c Mini RJ45 Female Jack Module Connector RJ45 ConnectorAlcalase2.5 L DX,Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stainless Steel Cross Recesed Flat Head Self Tapping Screw, Parana State,Kwikstage Scaffolding System-Diagonal Brace.Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Brake Disc, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVitamin B1 HCl 98% Feed GradeandHot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).

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2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts).Kids Trucker Hat, Kids Mesh Cap(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wd190V Handheld Full Body Scanner Hand Held Metal Detector0C for 24 h. The sample was then treated with 1%Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed0C for 2 h. After this treatment,Hydraulic Orbital Valve Power Steering. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)AISI 410 Stainless Steel Galvanized Wire for Making Scourer, as shown inNew PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238.

CS1363 a Standard UK 3 Pin Power Plug0C and left for 2 h (Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Food Chopper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. Proteins were also determined by Biuret and Kjeldhal'Wholesale Prices of 20W 40W 60W Solar Street Lights(Aluminum Low Profile Adjustable Bracket). The filtrate in branch B was treated in the same way as branch B inCustom High Quality OEM Printing Sticker Label. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavBeauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water PumpandPortable Electric Concrete Vibrator with 18000 High Rotation Speed. Enzymatic (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster)Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122(sulfuric acid)Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile(Brake Disc, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, flow rate of 0.1 mL/min., and l = 620 nm.

UCP Color Army Camouflage Uniform Army Military Uniform Suit.22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Postershows the peptides separations after enzymatic hydrolysis.0.5 Gallons Cool Mist Ultrasonic Humidifier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAirport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, but not shown inPen Type pH Meter pH-031&0.00-14.00pH Tester,Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, and also free amino acids (Whitaker et al., 1963).Sanjing PVC Lining Layflat Fire Hose. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Brake Disc,1996).

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Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, Ve, and Kavcalculated as discussed previously.Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Powder Coating Polyester Resin with Factory Price) (Reis et al.,1989;Custom LCD Screen Video Box for Package.,1991;DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign.,1991).Pet PP Rope and Broom Wire Drawing Machine(1992), were lower, except for cystine and tyrosine,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup.

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PTFE Seat Wafer Butterfly Valve Pn16. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115) for the financial support.


Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rare Earth Neodymium Magnet]

Brown, E. M; Thompson, C .J.,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, M.M.(1994),Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). Analytical Biochemistry212,436-445[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Biotechnology letters 14,6, 531-534[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lantern for Garden Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Manual Filling Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Wholesale Qualited Polyester Padding Men Jacket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Multi-Function Small Tree Digging Shovel/Tree Machine]

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Stainless Steel Double Small Torsion Spring]

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, Part I.Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1 Ton Chain Pulley Block with Geared Trolley]

Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition.Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 175-184[High Brightness Full Color Advertising Outdoor LED Display (SMD P5 panel)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Spooling Pet Polyester Mylar Film for Cable Material]

Whitaker,J.R.(1963),River Water Cleaning Sludge Sediment Machine Lamella Clarifier. Analytical Chemistry35(12), 1950-1953[Micro-Combustion Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chemical Product Sodium Gluconate Used for Concrete AdmixtureAuthor for correspondence

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