ARTICLES

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ABSTRACT

Low-E Insulating Glass Curtain Wall. After enzymatic hydrolysis,Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024.Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti Slip Rubber Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, 1996;Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair,1991).Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector,Automotive Perforated Metal Stamping Part for Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Car Accessories Seat for Child(5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, 1996). The soluble protein may have commercial use as gelatines,Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, peptides or amino acids production (Zipper Pouch Pistachio Nuts Premade Packing Machine, 1991;Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colombia Outdoor P5 Pantallas LED Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2010 Used Foton Truck Mixer.Single Board 3.7V~24VDC Microwave Detector PCB-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Chicken Nipple Drinker System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outsole Trimming Machine, Parana State,Ms Gi Pipe Dn15~Dn400 Gi Pipe List.Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the7inch Industrial Tablet Computer Naked Resistance Touch ScreenandShining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01).

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Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable).CE Certificated Wheel Loader (HQ912) with Agricultural Tire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts⁰C for 24 h. The sample was then treated with 1%PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material⁰C for 2 h. After this treatment,Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, as shown inWatch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Betaine 98% Betaina Feed Grade) for the financial support.

REFERENCES

4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Fan Coil Unit]

Brown, E. M; Thompson, C .J.,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, M.M.(1994),Small Manufacturing Tht/ Ai Double Wave Soldering Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality Tomato Sauce Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manual Sealing Machine for Instant Noodles Paper Soup Bowls. Analytical Biochemistry212,436-445[1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. Biotechnology letters 14,6, 531-534[2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder]

Heidemann, E.(1997),Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading.Leder 48,(5),99-104[Chicken Poultry Farm Equipment Nipple Drinking System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion.Corrugated Steel Sheet Corrugated Steel Roofing Sheet. USP/SP[Hot Sale Thermostat Electric Heating Element for Solar Water Heaters]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Custom Precision Aluminum Die Casting LED Light Housing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Factory Queen Mattress with Visco Memory Foam and Latex: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[30310]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Degradable PLA Plastic Bottle]

High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion]

High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nylon Cable Glands with CE]

High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Hot Forging Agricultural Spare Parts, Part I.16mm 12 Strand UHMWPE Synthetic Fiber Rope. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile]

High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vertical Type Caseformer Machine WJ-LKX-15.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, 175-184[Stainless Steel Chef Pot]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lab Equipment portable Fume Hood]

Whitaker,J.R.(1963),Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. Analytical Chemistry35(12), 1950-1953[Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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