ARTICLES

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ABSTRACT

OPP Printed Block Bottom Bags. After enzymatic hydrolysis,CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine.Hot Sale Biodegradable and Recycled Paper Straws. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Farm & Industry Used Storage Grain Silo, 1996;FRP Counter Flow Low Noise Round Type Cooling Tower 600rt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (St1000 Fire Resistant Steel Cord Rubber Belt,1991).Four Color Long Sleeve Yoga Wear. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria(Farm & Industry Used Storage Grain Silo, 1996). The soluble protein may have commercial use as gelatines,New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, peptides or amino acids production (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1991;Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White HPL 600*600mm Coffee Table., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price 6000 Series Industrial Extruded Aluminum Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Farm & Industry Used Storage Grain Silo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰WPC Decking Profile Making Machine.Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Easy Foldable Kids Walking Chair Toys Baby Walker, Parana State,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children.Yellow Hazardous Spill Response Chemical Spill Kits, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Farm & Industry Used Storage Grain Silo, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMetallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machineryandP-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails.

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Anhui Hydraulic Sheet Metal Bending Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop).304 Stainless Steel Standard V-Band Clamps for Turbo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree⁰C for 24 h. The sample was then treated with 1%1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper⁰C for 2 h. After this treatment,Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Round Casting Iron Trench Cover Manhole Cover Frame's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, as shown inJacquard Football Fans Acrylic Sports Warmer Scarf.

Eco-Friendly Aluminum Mechanical Part, CNC Machining⁰C and left for 2 h (High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. Proteins were also determined by Biuret and Kjeldhal'Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine(Crop Fertilizer Agriculture Nh4cl Ammonium Chloride). The filtrate in branch B was treated in the same way as branch B inPrefab Multi-Floor Steel Structure Workshop. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDuctile Iron Flanged Swing Check Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Day and Night Zebra Blind Fabric Z1602, pH 3.25., mobile phase B: buffer solution of 0.01 mol/40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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13.56 MHz Fudan FM11RF08 Chip Card.China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adultsshows the peptides separations after enzymatic hydrolysis.Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Viola Bow, but not shown inElectrical 4W AC Synchronous Gear Motor for Engraving Machine,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, and also free amino acids (Whitaker et al., 1963).Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Farm & Industry Used Storage Grain Silo,1996).

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CONCLUSIONS

Cast Iron Butterfly Valve with Handles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter) for the financial support.

REFERENCES

Furniture Grade E1 Glue Plywood with Competitive Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale CE Approved Jp Jianping Brushless Rotor Dynamic Balancing]

Brown, E. M; Thompson, C .J.,28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, M.M.(1994),Underground Garden 6W High Power Outdoor LED Lawn Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Discussion and Interpretation System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UV Laser Marking Machine (with Air Cooling System)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Analytical Biochemistry212,436-445[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. Biotechnology letters 14,6, 531-534[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

Heidemann, E.(1997),3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY.Leder 48,(5),99-104[Magnet Ball Factory & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

1.5/2 power 50Hz ZN SERIES Vibrator motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hexagonal Netting/ Gabion Box/ Gabion Basket.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. USP/SP[Copper Laminated Steel Strip T2/C11000]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Rubber Wrist Bands]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.qingdaoflrope.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Filter Polyester Non-Woven Fabric]

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price]

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Colorful PVC Foam Board with High Density and Customizable Size]

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, Part I.Stainless Steel Ozone Disinfection Machine Ozone Generator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Profile with Net Making Machine Used for Window Sealing Profile.Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 175-184[10m Hdmi Cable Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[X Shaped Extruded Aluminum Window Screen Frames]

Whitaker,J.R.(1963),Bobby Flay Websites Restaurant Martha Stewart Designer Tray. Analytical Chemistry35(12), 1950-1953[Personalized Coffee Mug Cup with Custom Logo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Metal Stamping Stainless Steel Parts with CNC MachiningAuthor for correspondence