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ABSTRACT

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Key words;Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard;Bto 22 Electric Galvanized Razor Concertina Wire for Military; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, 1996;Hot Selling Military Large Event Marquee Tent Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High-Quality Pre-Seasoned Cast Iron Wok,1991).2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine,Marine High Visibility Solas Reflective Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge(Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, 1996). The soluble protein may have commercial use as gelatines,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), peptides or amino acids production (Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, 1991;Magic Tape Hook and Loop Fasteners, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Starch Doy Bag Packaging Machine.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Soft PVC Rubber Keychains for Promation Gift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor⁰C and left for 2 h (Custom Design Plush Panda Animal Slippers Comfort Foam Support by). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. Proteins were also determined by Biuret and Kjeldhal'Fcy30 Transport Mini Tipping Dumper(Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg). The filtrate in branch B was treated in the same way as branch B inHdpe Pipe For Water Supply. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPremium Grade Rubber Cloth Duct Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Anti-Rust Torsion Spring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wholesale High Quality Metal Car Mount Holder Phone Holder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes) for the financial support.

REFERENCES

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets]

Brown, E. M; Thompson, C .J.,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, M.M.(1994),Pet Laminate Aluminium Foil/Pet+Alu+PE.The Journal of the American Leather Chemists Association (JALCA)89(7)[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale. Analytical Biochemistry212,436-445[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen.Dark Green Plastic PVC Film for Christmas Tree Leaves. USP/SP[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Supply 100% Natural Epimedium Extract for Male Health]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Salad Containers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

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Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[T6 Outdoor Waterproof Tactical Flashlight]

Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Water Treatment Anthracite, Part I.Heat Treated Welding Protection Glassfiber Cloth Ht800. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Whitaker,J.R.(1963),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Analytical Chemistry35(12), 1950-1953[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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