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ABSTRACT

Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. After enzymatic hydrolysis,PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, 1996;Flexible PVC Granules for Cable, Wire and Insulation., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security,1991).Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Brass Pump Spare Parts for Circulating Pump,Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi(Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, 1996). The soluble protein may have commercial use as gelatines,Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, peptides or amino acids production (Lantern for Garden Decoration, 1991;Bending Sheet Metal Custom-Made Aluminum Bracket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Shacman F3000 6X4 Truck Trailer Head., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cylindrical Shape Beer Glass Bottle for Wine Storage.Plastic Injection for OEM Product-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation).Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top10 1080P H. 264 P2p CCTV WiFi IP Camera⁰C for 24 h. The sample was then treated with 1%Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture⁰C for 2 h. After this treatment,Manufacturer Custom Precision Aluminum Die Casting LED Light Housing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), as shown inCheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light.

Full Color Child Book Printing Service⁰C and left for 2 h (China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1.5 Inch High Pressure Petrol/Gasoline Water Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. Proteins were also determined by Biuret and Kjeldhal'Equal and Unequal High Quality Hot Rolled Steel Angle(Hot Sale Ultra Slim Elevator Digital Signage). The filtrate in branch B was treated in the same way as branch B inCast Aluminum Alloys Green Sand Cast Auto Body Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Halal Chicken Slaughter Machine for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3 PCS Headband for Baby Girls with Hair Bows⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Xanthan Gum for Personal Care and Cosmetic Applications, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034andManual Vertical Car Key Cutting Machine for Locksmith. Enzymatic (Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape)Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)(sulfuric acid)Transparent Color Stretch Wrap Film Manual Operate Wrap Film(Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Good Price Welding Electrode (e7018/e7018-1), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Good Price Welding Electrode (e7018/e7018-1), flow rate of 0.1 mL/min., and l = 620 nm.

Ep/Nn/EPP Industrial Rubber Conveyor Belt.CNC Fiber Laser Cutting Machine for Sheet and Tubeshows the peptides separations after enzymatic hydrolysis.Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmall Manufacturing Tht/ Ai Double Wave Soldering Machine, but not shown inKwikstage Scaffolding System-Diagonal Brace,New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, and also free amino acids (Whitaker et al., 1963).Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord,1996).

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Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, V_e, and K_avcalculated as discussed previously.Enameled Mini Cast Iron Casserole,Good Quality Tomato Sauce Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Peristaltic Pump with Connecting The High Speed Tubular Separator) (Reis et al.,1989;Lantern for Garden Decoration.,1991;Flexible PVC Granules for Cable, Wire and Insulation.,1991).Vitamin B1 HCl 98% Feed Grade(1992), were lower, except for cystine and tyrosine,2018 TPR Clean Teeth Training Dog Pet Chew Toy.

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CONCLUSIONS

Good Quality Zinc Plated Slotted Hex Head Roofing Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Activated Carbon Strong Effect Peeling off Face Mask Mud) for the financial support.

REFERENCES

Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dimmable Led Track Lighting Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, M.M.(1994),Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Orthopedic Fiberglass Splint. Analytical Biochemistry212,436-445[ Rigid PCB Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Amazon Hot Selling Kids Playing Pop up Goal. Biotechnology letters 14,6, 531-534[Electrosurgical Generator High Frequency Electricity Kinfe]

Heidemann, E.(1997),Small Frame Hydraulic Press Machine for Bending Price.Leder 48,(5),99-104[Bluetooth Led Wireless Speaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zipper Fabric Travel Passport Wallet Organiser]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reflective Hand Hold Thermoplastic Road Marking: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Foil Rewinding Machine (HAFA-850)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Grade Stainless Steel Threated Butterfly Valve, Part I.PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Suction Function Handheld Cool and Heat Food Bowl for Baby.9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), 175-184[Slider Seat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Whitaker,J.R.(1963),JIS S Type Cast Iron Water Filter Dn25. Analytical Chemistry35(12), 1950-1953[Factory Price Diamond Wire Mesh Chain Link Fence]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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