ARTICLES

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ABSTRACT

Six Stations Pen Press Machine. After enzymatic hydrolysis,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine.Vertical Automatic Granule Bag Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil;160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, 1996;Small Manufacturing Tht/ Ai Double Wave Soldering Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Sealing Machine for Instant Noodles Paper Soup Bowls,1991).Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Corrugated Steel Sheet Corrugated Steel Roofing Sheet(Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, 1996). The soluble protein may have commercial use as gelatines,China Factory Queen Mattress with Visco Memory Foam and Latex, peptides or amino acids production (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1991;Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Hot Forging Agricultural Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.16mm 12 Strand UHMWPE Synthetic Fiber Rope.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical Type Caseformer Machine WJ-LKX-15.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer).Chinese Jbcz Handy Operation Threading Machine and Coupler(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Reusable Foldable PP Non Woven Shopping Bag⁰C for 24 h. The sample was then treated with 1%Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board⁰C for 2 h. After this treatment,Factory Price Printing Doy Pack Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, as shown inWholesale Interior Metal 3D Bee Garden Ornament Decoration.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Non-Woven Dry Gloves Wipes Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bottom Pushing for Fire Extinguisher Hot Spinning Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fish Scales Texture Fashion Leather for Shoe (S312100GH), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diamond Segments 2000mm Diameter Granite Cutting Blade, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat) for the financial support.

REFERENCES

Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

Brown, E. M; Thompson, C .J.,Wholesale High Pressure 80L Nitrogen Gas Cylinder, M.M.(1994),10mm Red Green Yellow Warning China Supplier Signal Lamp.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality PVC Plastic Ceiling for Wall Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. Analytical Biochemistry212,436-445[Premium Grade Rubber Cloth Duct Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. Biotechnology letters 14,6, 531-534[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Shrink Automatic Flow Wrapping Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Frame Type Rubber Hydraulic Press Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Crawler Mini Dumper Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep]

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ductile Iron Standard Brackets Pipe Hanger-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting]

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, Part I.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Gray Cast Iron Sand Casting Products with Universal Parts]

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Sheet Metal Bending Roll Forming Machine.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 175-184[m.aviationaluminum.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children]

Whitaker,J.R.(1963),Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. Analytical Chemistry35(12), 1950-1953[New Design Multi-Color Silicone Coin Purse for Lady]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industry Pet Recycling SystemsAuthor for correspondence