ARTICLES

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ABSTRACT

Women Summer Swimsuit Chest Zip Bathing Suit. After enzymatic hydrolysis,Hemp Seed Oil Body Lotion Moisturizes The Skin.SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal;UTP CAT6 48 Ports Vertical Termination Patch Panel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Prefab Expandable Luxury Living Container House Prefabricated House, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1996;Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close,1991).Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Hexagonal Wire Mesh Gabion / Gabion Box,10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Must 4kw 2HP Pump Power Inverter Ags Bts Available(Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Small Steel Silo for Poultry Feed, peptides or amino acids production (Good Price Nasal Paste Packaging Machine, 1991;Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight.Barbed Wire Right off The Border Wall to Protect Homes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), Parana State,Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.Fashion Design Underwear for Men Cotton Mens Boxer Briefs, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1.5 Inch High Pressure Petrol/Gasoline Water PumpandFarm & Industry Used Storage Grain Silo.

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Nylon Balance Bike with Water Transfer Printing Malaysia(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes).Anti Slip Rubber Mat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap⁰C for 24 h. The sample was then treated with 1%Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor⁰C for 2 h. After this treatment,Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, as shown inFish Scales Texture Fashion Leather for Shoe (S312100GH).

Automotive Perforated Metal Stamping Part for Furniture⁰C and left for 2 h (Automatic Chicken Nipple Drinker System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Car Accessories Seat for Child. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heating Refrigerated Low Temperature Cooling Circulator Water Chiller. Proteins were also determined by Biuret and Kjeldhal'Zipper Pouch Pistachio Nuts Premade Packing Machine(Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle). The filtrate in branch B was treated in the same way as branch B inEuropean Travelling Trolley Electrical Hoist. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColombia Outdoor P5 Pantallas LED Display(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2010 Used Foton Truck Mixer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Board 3.7V~24VDC Microwave Detector PCB, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Chicken Nipple Drinker System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)andGood Quality Good Price China Plastic Injection Molding Machineshow the gel chromatographic separation of soluble protein with Sephadex G-PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material.Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet, and another peak at 45 mL not shown inVertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, for peptides lower than 3000 Da.Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump(Condell et al.,1993) .

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ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, V_e, and K_avcalculated as discussed previously.Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100),Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality) (Reis et al.,1989;Good Price Nasal Paste Packaging Machine.,1991;Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries.,1991).DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw(1992), were lower, except for cystine and tyrosine,Auto Two Piece Folder Gluer for Corrugated Box.

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CONCLUSIONS

British Standard 2 Gang 1 Way Wall Light Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low-E Insulating Glass Curtain Wall) for the financial support.

REFERENCES

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hdmi Audio Connector]

Brown, E. M; Thompson, C .J.,Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, M.M.(1994),Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Coal Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Small Steel Silo for Poultry Feed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier. Analytical Biochemistry212,436-445[Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory]

Dalev, P.G. and Simeonova, L.S.(1992),Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. Biotechnology letters 14,6, 531-534[Cylindrical Shape Beer Glass Bottle for Wine Storage]

Heidemann, E.(1997),P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails.Leder 48,(5),99-104[Big Tin Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat Pump Non-Programmable HVAC Room Thermostat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electronic Repair Tool]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo]

6.6kw LiFePO4 Charger for Lithium Battery Pack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Explosion Proof Fog Cannon for Coal Plant]

6.6kw LiFePO4 Charger for Lithium Battery Pack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

6.6kw LiFePO4 Charger for Lithium Battery Pack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50m Depth Long Range Metal Detector with Reliable Quality, Part I.CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Stable Burn-in Test Equipment.Wholesale Custom 100% Cotton Boys T-Shirt with Print, 175-184[Copper Arched Fishing Flexible Floor Lamp for Living Room Dinning Room]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Whitaker,J.R.(1963),Industry 0.002mm Accuracy Checking Fixture. Analytical Chemistry35(12), 1950-1953[Alumina Ball with High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automotive Water Pump SealAuthor for correspondence