ARTICLES

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ABSTRACT

Best Design and Nice Price All in One Solar Street Light. After enzymatic hydrolysis,Customized Large Ss Stamping/ Bending Sheet Metal Parts.High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, 1996;Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle,1991).Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve,Polished Tzm Molybdenum Alloy Plate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Iron Welded Gabion Basket Retaining Wall(Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, 1996). The soluble protein may have commercial use as gelatines,Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, peptides or amino acids production (Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale, 1991;Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FTTX Solution Provider Fiber Optical 12cores MPO Cassette., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Height Adjustable Cane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board.Large Batches Equipoise Light Yellow Liquids-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Spraying Drying Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Humic Acid Bio-Fertilizer Granule Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Load 1600kg Small Machine Room Bed Elevator) for the financial support.

REFERENCES

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Brown, E. M; Thompson, C .J.,Low Frequency 3000W 24V 230V Inverter with Battery Charger, M.M.(1994),Amazon Best Selling Sports Ankle Support Sleeves Compression.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pre-Engineered Cage Broiler Poultry Farm Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pen Type pH Meter pH-031&0.00-14.00pH Tester. Analytical Biochemistry212,436-445[Amazon Hot Selling Kids Playing Pop up Goal]

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Heidemann, E.(1997),ASTM Iron Sand Casting Parts.Leder 48,(5),99-104[Forged & Machined Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Bar Peeling Lathe Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Approved Sperm Concentration Test for Sperm Density Test: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pipe Sheet Laser Cutter with Tube Cutting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale 7.2X4.5X4.7m Christmas Inflatable Santa Bouncer with Slide]

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Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Screen Display Wireless Charging 10000mAh Power Bank]

Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck]

Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sand Casting Parts Large Size for Industrial Use, Part I.Durable Steel Retainer Plastic Road Safety Trench Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pearls Flake 99% Factory Naoh Caustic Soda]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

Whitaker,J.R.(1963),Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts. Analytical Chemistry35(12), 1950-1953[Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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