ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fire Extinguisher UV Curing Machine Cylinder Drying Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set

ABSTRACT

Energy-Saving Biogas Double Stove for Sale. After enzymatic hydrolysis,Valve Bag Dry Mortar Bagging Machine.High Quality 500m 100lb Super Strong Braided Fishing Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1996;Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending,1991).Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Stainless Steel Jewelry Production by Metal Injection Molding Process,Factory Directly Selling Engine Standard Spark Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Logo Silicone Wristband, Rubber Wrist Bands(PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1996). The soluble protein may have commercial use as gelatines,Custom All Kinds of Acrylic Shoe Display Racks, peptides or amino acids production (Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, 1991;Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Supply U40 Disposable Insulin Syringe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Water Chiller Air Cooled Chiller Industrial Chiller for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair.High Precision Tungsten Carbide Machine Customized Cutting Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Maleic Anhydride CAS 108-31-6Alcalase2.5 L DX,2000mj Q Switched for Pigment Removal with Ce Certification, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cow Dung Chicken Manure Organic Fertilizer Making Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

marble Makeup Brush Set for Travel in 20PCS, Parana State,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set.Swimming Pool Water Disinfect Ozone Generator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the120W Ultra Light Solar Blanket Panel for CampingandNovel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould.

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20kv 50kVA High Voltage Dry Type Power Supply Transformer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse).Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter⁰C for 24 h. The sample was then treated with 1%4 Styles Blue Glass Crystal Butt Beads Spiral Plug⁰C for 2 h. After this treatment,Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Activated Carbon Water Filter Industry Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2016 New Generation Sausage Making Machine, as shown inExcavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)⁰C and left for 2 h (Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03). Proteins were also determined by Biuret and Kjeldhal'China Supplier Building Construction Steel Frame (ZY431)(1.5/2 power 50Hz ZN SERIES Vibrator motor). The filtrate in branch B was treated in the same way as branch B inwww.newayt.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lab Equipment portable Fume Hood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cracker Multi Rows Trayless Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured) for the financial support.

REFERENCES

4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.flsygroup.com]

Brown, E. M; Thompson, C .J.,22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, M.M.(1994),Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bluetooth Smart Watch with Heart Rate Monitor for Men 72087]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Marine High Visibility Solas Reflective Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Power Consumption Laser Foam Vertical Die Cutting Machine. Analytical Biochemistry212,436-445[Maleic Anhydride CAS 108-31-6]

Dalev, P.G. and Simeonova, L.S.(1992),New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug. Biotechnology letters 14,6, 531-534[High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes]

Heidemann, E.(1997),Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse.Leder 48,(5),99-104[Battery Operated Fabric Shaver]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Acm Dewatering Twin Screw Extruder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Professional Mold Factory Plastic Injection Mould Maker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SGS Direct Thermal Label for Supermarket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[No Smoke Rice Husk Continuous Type Carbonization Stove for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds]

High Quality Crusher for Used Plastic Recycling Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Cheap Portable Stage Aluminum Truss Stage]

High Quality Crusher for Used Plastic Recycling Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine]

High Quality Crusher for Used Plastic Recycling Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, Part I.Wear Resitant Rope End Cap Plastic Flange Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material]

Comfortable Two Piece Women Fitness Clothing for Gym Wear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner.Flat Surface Mounted 300mm 24W LED Panel Light for Interior, 175-184[Bath Mesh Sponge Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Silicone Rubber Electronic Cable with High Flexibility]

Whitaker,J.R.(1963),Latex Paint Emulsion Thickener Agents with High Leveling Property. Analytical Chemistry35(12), 1950-1953[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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