ARTICLES

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ABSTRACT

UCP Color Army Camouflage Uniform Army Military Uniform Suit. After enzymatic hydrolysis,0.5 Gallons Cool Mist Ultrasonic Humidifier.Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sanjing PVC Lining Layflat Fire Hose;Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic CNC Glass Cutting Machine Glass Cutting Table, 1996;Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken,1991).Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pet PP Rope and Broom Wire Drawing Machine,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PTFE Seat Wafer Butterfly Valve Pn16(Automatic CNC Glass Cutting Machine Glass Cutting Table, 1996). The soluble protein may have commercial use as gelatines,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, peptides or amino acids production (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 1991;Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plain Makeup Bag Canvas Cosmetic Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic CNC Glass Cutting Machine Glass Cutting Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment).River Water Cleaning Sludge Sediment Machine Lamella Clarifier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polyester Coated Stainless Steel Ladder Type Metal Ties⁰C for 24 h. The sample was then treated with 1%2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device⁰C for 2 h. After this treatment,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Copper Laminated Steel Strip T2/C11000's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Halal Empty Gelatin Capsule for Medicine, as shown inMattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm⁰C and left for 2 h (Best Selling Household Living Room Mat Chenille Carpets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gym Fitness Exercise Sporting Body Building Bicep Press. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. Proteins were also determined by Biuret and Kjeldhal'Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making). The filtrate in branch B was treated in the same way as branch B inCopper Coated Mesh Scourer Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIntegrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic ToysandChuke 20W Optical Fiber Laser Marking Machine Printer Engraver. Enzymatic (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank)Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(sulfuric acid)Plastic Food Box Container Covers by Injection Mould Mold Tool(Automatic CNC Glass Cutting Machine Glass Cutting Table, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans.Wvrb Series Planetary Gearbox Used for Robotshows the peptides separations after enzymatic hydrolysis.Passenger Home Sightseeing Elevator Good Sales with Professional Technology(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, but not shown inBluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,Vacuum Stainless Steel Casting Products, and also free amino acids (Whitaker et al., 1963).Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic CNC Glass Cutting Machine Glass Cutting Table,1996).

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CONCLUSIONS

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam) for the financial support.

REFERENCES

Chunke Portable UV Water Sterilizer for Water Purifier 150t, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nail Luminous Pigment]

Brown, E. M; Thompson, C .J.,Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, M.M.(1994),Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality NBR70 O-Ring with Reasonable Prices]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Xjm Series Rubber Extruding Machine. Analytical Biochemistry212,436-445[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

Dalev, P.G. and Simeonova, L.S.(1992),Stop Dog From Jumping or Climbing Your Chain Link Fence.. Biotechnology letters 14,6, 531-534[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

Heidemann, E.(1997),SGS Direct Thermal Label for Supermarket.Leder 48,(5),99-104[Medical Products Non-Absorbable Surgical Suture Polypropylene Monofilament PP]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine]

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Water Filter Polyester Non-Woven Fabric.Industry Pet Recycling Systems, Industry Pet Recycling Line. USP/SP[I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sublimation Polyester Sports Tshirt for Adult: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Skytone 4 Channels 3500W Each DJ Power Amplifier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chicken Pressure Fryer Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Heavy Duty Marquee Advertising Pop up Tent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic L Bar Sealer Shrink Tunnel Machine]

Heavy Duty Marquee Advertising Pop up Tent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Common Rail Diesel Injector Nozzle Dlla153p977]

Heavy Duty Marquee Advertising Pop up Tent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fire Extinguisher UV Curing Machine Cylinder Drying Machine, Part I.Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polyurethane Gasket Foam Seal Dispensing Machine]

Energy-Saving Biogas Double Stove for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Valve Bag Dry Mortar Bagging Machine.High Quality 500m 100lb Super Strong Braided Fishing Line, 175-184[m.yycj.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

Whitaker,J.R.(1963),20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Analytical Chemistry35(12), 1950-1953[Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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