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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label

ABSTRACT

Men Fashion Plaid Design Polyester Ties (WH24). After enzymatic hydrolysis,70m Series Aluminiun Plastic Coated Profile for Widnows.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vertical Type Automatic Box Erector Machine;Air Cooled Scroll Type Water Chiller for Industrial Process; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, 1996;Rotary Vibrating Sieve Machine for Compost., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud,1991).Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa,Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube(Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, 1996). The soluble protein may have commercial use as gelatines,Condoms/Sex Toys/Capsule Toy Vending Machine, peptides or amino acids production (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), 1991;Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Type 40kgs Movable Ice Maker for Store Use., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric 100W Three Wheels Toy Car for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2*2 4G Lte MIMO Antenna Rrouter/CPE.2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electric Galvanized Coated Barbed Wire (MT-BW013)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cute Clear Fanny Pack Stadium Security PVC Waist Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Silicone Reusable Food Storage Bag China Manufacturer).Personalized Coffee Mug Cup with Custom Logo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears⁰C for 24 h. The sample was then treated with 1%10 Pieces Customized Professional Makeup Brush⁰C for 2 h. After this treatment,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrophoresis Assembly Infilltration Tank for Effluent Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)700mm RP HP UHP High Quality Graphite Electrode, as shown inCast Iron Butterfly Valve with Handles.

Prefab Building Insulated Interior Concrete Partition Walls⁰C and left for 2 h (Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Disk Filter System with Automatic Backwash Valves. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Eco-Friendly Recycling PS Foam Container Production Line. Proteins were also determined by Biuret and Kjeldhal'Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve(U. S. Type Drop Forged Wire Rope Clip). The filtrate in branch B was treated in the same way as branch B in3 Bearings Pedal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJacquard Football Fans Acrylic Sports Warmer Scarf(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Blinking Battery Operated Luminary Plastic LED Tea Lights⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion Men′s Clothing Shop Interior Design Clothes Store FixturesandUs Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. Enzymatic (High Precision Plastic Mold Manufacturer in China)Mango Drying Machine/Solar Fruit Drying Machine(sulfuric acid)Mineral Industry Magnet Ore Ball Milling Machine(Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut, flow rate of 0.1 mL/min., and l = 620 nm.

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)shows the peptides separations after enzymatic hydrolysis.Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, but not shown inWholesale All Kinds Cheap Plush Animal Baby Slippers,Blasting Cleaning Shot Peeling Machine Tumble Belt Type, and also free amino acids (Whitaker et al., 1963).B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090,1996).

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CONCLUSIONS

Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester) for the financial support.

REFERENCES

Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Non-Ionic Polyacrylamide Flocculant]

Brown, E. M; Thompson, C .J.,Good Quality PVC Plastic Ceiling for Wall Panel, M.M.(1994),Heavy Steel Structures for Power, Cement, Coal Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Machining Metal/Aluminum/Brass Auto Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Manufacture Commercial Use Spice Grinder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+. Analytical Biochemistry212,436-445[2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo]

Dalev, P.G. and Simeonova, L.S.(1992),CE Approved Manual Heat Press Machine. Biotechnology letters 14,6, 531-534[Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Korean Integrated CTO Activated Carbon Filter for Water Treatment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.5 Gallons Cool Mist Ultrasonic Humidifier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Exterior Heavy Duty Locking Gate Hinges with 2 Ball Bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional Customized PP Hand Fan]

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160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, Part I.600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chemical Plant Transfer Industrial Water Pump]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital]

Whitaker,J.R.(1963),Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Analytical Chemistry35(12), 1950-1953[Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

60m3/H Lamella Clarifier for River Water TreatmentAuthor for correspondence