ARTICLES

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ABSTRACT

Enameled Mini Cast Iron Casserole. After enzymatic hydrolysis,Good Quality Tomato Sauce Making Machine.Vitamin B1 HCl 98% Feed Grade. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, 1996;Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Orthopedic Fiberglass Splint,1991).Amazon Hot Selling Kids Playing Pop up Goal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Frame Hydraulic Press Machine for Bending Price,3.5-7.0g Ozone Generator Purification ---Commercial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Baby Feeding Chair Plastic Chair(5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, 1996). The soluble protein may have commercial use as gelatines,Reflective Hand Hold Thermoplastic Road Marking, peptides or amino acids production (Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), 1991;Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Stainless Steel Threated Butterfly Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Suction Function Handheld Cool and Heat Food Bowl for Baby.9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:JIS S Type Cast Iron Water Filter Dn25(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W, Parana State,Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday.Worm Gear Fluorine Lined Butterfly Valve Flanged Type, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustry Pet Recycling SystemsandCommercial Crossfit Fitness Gym Equipment/Home Gym Equipment.

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H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Prices of 20W 40W 60W Solar Street Lights).Poultry House, Livestock, Poultry Farm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine⁰C for 24 h. The sample was then treated with 1%Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2⁰C for 2 h. After this treatment,Black Slim PU Leather Passport Travel Wallet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, as shown in Kids Mesh Cap.

3 Colors Tactical Shooting Hunting Camouflage Clothes Suit⁰C and left for 2 h (Slotter Sheet Metal V Grooving Machine From Professional Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1.3KG Fire breaker for kitchen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign. Proteins were also determined by Biuret and Kjeldhal'Korean Integrated CTO Activated Carbon Filter for Water Treatment(Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve). The filtrate in branch B was treated in the same way as branch B inYangtze Positive Tolerance on Grid Solar Panel Power Station Roof Mount System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vacuum Squeeze Filter Press for Coal Flotation Tailings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modular Portable Shipping Container Lkit Building Home Villa House. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wheel-Type-Carriage Precision Heavy Duty Linear Guide, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Boned Polyester Woven Faux Suede Sofa Fabric, V_e, and K_avcalculated as discussed previously.Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making,20 Bottles Electric Wine Display Cooler. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress) (Reis et al.,1989;Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).,1991;Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer.,1991).High-Efficient Htk Walnut Shell Filter for Water Purification(1992), were lower, except for cystine and tyrosine,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576).

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CONCLUSIONS

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L) for the financial support.

REFERENCES

Popular European Style White Marble Coffee Tables for Dinnig Room, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized Industry Steel Structure Staircases for Floor]

Brown, E. M; Thompson, C .J.,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, M.M.(1994),FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Energy-Saving Biogas Double Stove for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Kwikstage Scaffolding System-Diagonal Brace. Analytical Biochemistry212,436-445[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Dalev, P.G. and Simeonova, L.S.(1992),360 Rotating Automatic Touchless Car Wash Equipment with Drying System. Biotechnology letters 14,6, 531-534[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Heidemann, E.(1997),48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.Leder 48,(5),99-104[Factory Sales Potato Dehydrating Equipment/Fruit Slice Dehydrator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Taiding Custom Tank Sheet Metal Fabrication]

vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. USP/SP[BS 5163 Ductile Iron Rising Stem Gate Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sell Rubber / EVA / PE/PVC Foam Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.skyhammertools.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Brake Disc-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4-14 Manual Staple Gun with Staples for Hand Tools]

Sodium Gluconate 98% Min Industry Grade for Chemical Admixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign, Part I.24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder.LED Screen Display Wireless Charging 10000mAh Power Bank, 175-184[Metal Cabinet Drawer Slide]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

Whitaker,J.R.(1963),25kg 50kg Plastic PP Woven Feed Bags. Analytical Chemistry35(12), 1950-1953[Single Board 3.7V~24VDC Microwave Detector PCB]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cao Content Plastic Additives Desiccant Masterbatch for Recycled PlasticAuthor for correspondence