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ABSTRACT

13.56 MHz Fudan FM11RF08 Chip Card. After enzymatic hydrolysis,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Square Premium Glass Perfume Bottle 100ml, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, 1996;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep,1991).Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Inflatable PVC Flocking Health Bed,Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cast Iron Butterfly Valve with Handles(Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, 1996). The soluble protein may have commercial use as gelatines,Furniture Grade E1 Glue Plywood with Competitive Price, peptides or amino acids production (28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, 1991;Underground Garden 6W High Power Outdoor LED Lawn Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stamping Metal Parts Manufacturer Sheet Parts Fabrication., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hexagonal Netting/ Gabion Box/ Gabion Basket.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, Parana State,Top Quality Galvanized Iron Wire as Binding Wire.PVC Profile with Net Making Machine Used for Window Sealing Profile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePromotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthdayand380mm Working Area Concrete Diamond Floor Edge Grinder.

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Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PRO-Installer Fiber Optic Termination Kit Lk-6016).Bobby Flay Websites Restaurant Martha Stewart Designer Tray(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line⁰C for 24 h. The sample was then treated with 1%Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber⁰C for 2 h. After this treatment,Diameter 2.68m Paragraphed Autoclave for AAC Bricks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Travelling Weekender Mens Canvas Duffle Gym Sports Bag, as shown in48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.

4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box⁰C and left for 2 h (Flat Surface Mounted 300mm 24W LED Panel Light for Interior). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Selling Household Living Room Mat Chenille Carpets. Proteins were also determined by Biuret and Kjeldhal'130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(Warm Women Suede Leisure Snow Boots for Winter (FF-1)). The filtrate in branch B was treated in the same way as branch B inHot Sale Motorcycle Spare Parts Piston Ring Kit for YAMAHA Fz16. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Size Outdoor Store Front Signs Advertising Billboard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/T6s 86 PCD Core Bit for Geotechnical Drilling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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FSTN Type Monochrome Graphic Display Cog LCD with Backlight, V_e, and K_avcalculated as discussed previously.3D Embossed Iron on Label Clothing Heat Transfer Label,Glass Food Jar & Plastic Lid. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Nh4cl White Granular Agricultural Grade Ammonium Chloride) (Reis et al.,1989;28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.,1991;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.,1991).0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer(1992), were lower, except for cystine and tyrosine,Easy Operation Low Investment Cassava Silo.

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CONCLUSIONS

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain) for the financial support.

REFERENCES

100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Packaging Tea Tin Box for Chocolate New Fancy Christmas Gift Box Manufacturer Rose Gold Mickey Mouse 250g Metal Box]

Brown, E. M; Thompson, C .J.,Building Material High Quality 316 Stainless Steel Grating, M.M.(1994),Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. Analytical Biochemistry212,436-445[Halal Chicken Slaughter Machine for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Biotechnology letters 14,6, 531-534[ Wire and Insulation]

Heidemann, E.(1997),Quality Shaper Machine Bc6085 Shapping Machine.Leder 48,(5),99-104[High Quality PVC Coated Welded Wire Mesh Fence Panels and Galvanized Welded Wire Mesh]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Thermoplastic Road Marking Paint Glass Beads.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. USP/SP[Electric Galvanized Coated Barbed Wire (MT-BW013)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Resin Polishing Pad]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Party Mask LED Fiber Dance Princess Feather Mask]

I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ductile Iron Standard Brackets Pipe Hanger]

I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Free Sample! AC Power Contactor for Sale, Part I.PA System Loudspeaker 100W Outdoor Column Speaker . The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Breathable Surgical Disposable 3 Ply Earloop Face Mask.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, 175-184[Wholesale Price Brazilian Virgin Hair Lace Frontal for Black Women]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Whitaker,J.R.(1963),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. Analytical Chemistry35(12), 1950-1953[UV Coated PVC Panel for Interior Wall Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel PartsAuthor for correspondence