ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0.1mm Tungsten Carbide 2f Single Flute Micro End Mill

ABSTRACT

Agriculture Large Span Commerical Steel Frame Greenhouse. After enzymatic hydrolysis,Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ss400 Corner Metal Sheet Bar Galvanized Angle Iron;High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chinese Jbcz Handy Operation Threading Machine and Coupler, 1996;Promotional Reusable Foldable PP Non Woven Shopping Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board,1991).Factory Price Printing Doy Pack Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price(Chinese Jbcz Handy Operation Threading Machine and Coupler, 1996). The soluble protein may have commercial use as gelatines,High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, peptides or amino acids production (Engine Parts Rubber High Quality Timing Belt 111ru25, 1991;Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Non-Woven Dry Gloves Wipes Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chinese Jbcz Handy Operation Threading Machine and Coupler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65.Bottom Pushing for Fire Extinguisher Hot Spinning Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fish Scales Texture Fashion Leather for Shoe (S312100GH)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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All Weather Rattan Handwoven Swing Outdoor Home(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ultrasonic Automatic Face Mask Blank Making Machine).Pond Self Priming Booster suction  Water Pump with Pressure Switch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter⁰C for 24 h. The sample was then treated with 1%Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace⁰C for 2 h. After this treatment,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), as shown inOffice Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding.

Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle⁰C and left for 2 h (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. Proteins were also determined by Biuret and Kjeldhal'125kHz RFID Hotel Key Card with T5577 Chip(Top Quality Galvanized Iron Wire as Binding Wire). The filtrate in branch B was treated in the same way as branch B inOEM Face Anti-Aging Serum. The final soluble protein shown were used for peptides separations using gel permeation chromatography,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCrop Fertilizer Agriculture Nh4cl Ammonium Chloride(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10mm Red Green Yellow Warning China Supplier Signal Lamp⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Alumina Balls for Hydogen PeroxideandPhillip Countersunk Head Screw Hot DIP Galvanized. Enzymatic (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse)Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll(sulfuric acid)Heat Shrink Automatic Flow Wrapping Packing Machine(Chinese Jbcz Handy Operation Threading Machine and Coupler, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, flow rate of 0.1 mL/min., and l = 620 nm.

Ductile Iron Standard Brackets Pipe Hanger.Factory Stock Spring Steel U Type Clip Nutshows the peptides separations after enzymatic hydrolysis.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, but not shown in29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, and also free amino acids (Whitaker et al., 1963).5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chinese Jbcz Handy Operation Threading Machine and Coupler,1996).

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Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull HandleandProfessional Stainless Steel Ethanol Processing Plant From Cassavashow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Sheet Metal Bending Roll Forming Machine.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, and another peak at 45 mL not shown inMagic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys, for peptides lower than 3000 Da.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(Condell et al.,1993) .

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Frame Type Rubber Hydraulic Press Machine, V_e, and K_avcalculated as discussed previously.Kitchen Metal Dish Storage Rack (JP-SC984C),0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns) (Reis et al.,1989;Engine Parts Rubber High Quality Timing Belt 111ru25.,1991;Promotional Reusable Foldable PP Non Woven Shopping Bag.,1991).AVR Se350 Automatic Voltage Regulator for Marathon Alternator(1992), were lower, except for cystine and tyrosine,Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.

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CONCLUSIONS

Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Furring Channel Fittings Suspended Ceiling Accessories) for the financial support.

REFERENCES

High Strength Rigid PVC Plastic Foam Board for FRP, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food Additive Natamycin Factory & Manufacturer]

Brown, E. M; Thompson, C .J.,Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, M.M.(1994),4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Glass Food Jar & Plastic Lid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. Analytical Biochemistry212,436-445[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Dalev, P.G. and Simeonova, L.S.(1992),Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. Biotechnology letters 14,6, 531-534[Prefab Steel Structure Shed for Storage Warehouse]

Heidemann, E.(1997),LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.Leder 48,(5),99-104[Round Glass Jars Food Grade for Honey Jars Canned Fruit Vegetables Jars]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck. USP/SP[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rebonded Foam Machina]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality 100% PP Wrapping Film for Lady Sanitary Pad Usage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vitamin B1 HCl 98% Feed Grade]

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, Part I.High Quality Ceramic Chrome Hybrid Bearing Oil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder.Digger Arm Small Wheel Loader (HQ908) with CE, 175-184[En 10217-1 P235tr1 Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Whitaker,J.R.(1963),15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. Analytical Chemistry35(12), 1950-1953[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Low Profile Adjustable BracketAuthor for correspondence