ARTICLES

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ABSTRACT

China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). After enzymatic hydrolysis,Spine Shape Landscaping Artificial Turf Grass.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board;Diesel Engine Type Zzs Sand Vibrating Screen; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, 1996;Trendy High Quality 925 Stering Silver Tennis Glod Charm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites,1991).Stand 2.5t Electric Pallet Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion,Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, 1996). The soluble protein may have commercial use as gelatines,Men Fashion Plaid Design Polyester Ties (WH24), peptides or amino acids production (70m Series Aluminiun Plastic Coated Profile for Widnows, 1991;New Arrival Wooden Kids Cleaning Set Toy for Education W10d251, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical Type Automatic Box Erector Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Air Cooled Scroll Type Water Chiller for Industrial Process.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.Rotary Vibrating Sieve Machine for Compost-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Condoms/Sex Toys/Capsule Toy Vending Machine, Parana State,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Aluminium Windows Thermal Break China SupplierandZw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker.

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Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122).Outdoor Type 40kgs Movable Ice Maker for Store Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts⁰C for 24 h. The sample was then treated with 1%Electric 100W Three Wheels Toy Car for Sale⁰C for 2 h. After this treatment,2*2 4G Lte MIMO Antenna Rrouter/CPE. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Galvanized Coated Barbed Wire (MT-BW013), as shown inAquarium Air Pump with 2 Nozzles Adjustable Gas Flow.

Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging⁰C and left for 2 h (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Proteins were also determined by Biuret and Kjeldhal'Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool(DIN Standard Gratings/Gavlanized Steel Grating). The filtrate in branch B was treated in the same way as branch B inm.tysonleather.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCute Clear Fanny Pack Stadium Security PVC Waist Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10 Pieces Customized Professional Makeup Brush, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve.En1092-1 05 Pn16 Blind Flat Face Flangeshows the peptides separations after enzymatic hydrolysis.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, but not shown inCustomized Hot Forging Agricultural Spare Parts,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, and also free amino acids (Whitaker et al., 1963).Jacquard Football Fans Acrylic Sports Warmer Scarf. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bluetooth Smart Watch with Heart Rate Monitor for Men 72087,1996).

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CONCLUSIONS

DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet) for the financial support.

REFERENCES

Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.dksportbot.com]

Brown, E. M; Thompson, C .J.,Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump, M.M.(1994),Blasting Cleaning Shot Peeling Machine Tumble Belt Type.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine. Analytical Biochemistry212,436-445[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap. Biotechnology letters 14,6, 531-534[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

Heidemann, E.(1997),Chemical Plant Transfer Industrial Water Pump.Leder 48,(5),99-104[Ultrasonic Seamless Underwear Bra Lace Sewing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

Electric Wine Bottle Opener K020, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Insecticidal Lamp with Solar Energy Frequent Vibration.Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286. USP/SP[2018 TPR Clean Teeth Training Dog Pet Chew Toy]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Latex Paint Emulsion Thickener Agents with High Leveling Property]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silicone LED Tube Flex 12V LED Flexible Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Galvanized Square Washer with Timber Constructions, DIN436DIN436, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Do Not Enter Tape Warning Tape Caution Tape Danger Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Galvanized Square Washer with Timber Constructions, DIN436DIN436,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Galvanized Square Washer with Timber Constructions, DIN436DIN436, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), Part I.Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins]

Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality PVC Plastic Ceiling for Wall Panel.Heavy Steel Structures for Power, Cement, Coal Plant, 175-184[10mm Hole Size API J55/N80 Perforated Steel Casing Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Humizone Seaweed Customized Organic Liquid Fertilizer]

Whitaker,J.R.(1963),Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+. Analytical Chemistry35(12), 1950-1953[ Baby Finger Toothbrush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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