ARTICLES

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ABSTRACT

Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. After enzymatic hydrolysis,Galvanized Square Washer with Timber Constructions, DIN436DIN436.Do Not Enter Tape Warning Tape Caution Tape Danger Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm);Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality PVC Plastic Ceiling for Wall Panel, 1996;Heavy Steel Structures for Power, Cement, Coal Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+,1991).CE Approved Manual Heat Press Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820),High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets(Good Quality PVC Plastic Ceiling for Wall Panel, 1996). The soluble protein may have commercial use as gelatines,OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, peptides or amino acids production (160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, 1991;China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nbm High Quality Auto Part Factory Price 6pk2310. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality PVC Plastic Ceiling for Wall Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ultrasonic Automatic Face Mask Blank Making Machine.Manufacturer of Soda Ash Dense 99 2% Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle).2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine⁰C for 24 h. The sample was then treated with 1%Oak Laminate Grey Floating Cork Vinyl Plank Flooring⁰C for 2 h. After this treatment,China Manufacturer Customized Microwave Ring Ferrite Magnet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody, as shown inHigh Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.

Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker⁰C and left for 2 h (Hot Sale Electric LED light Aroma Diffuser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electrical 4W AC Synchronous Gear Motor for Engraving Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Car Seat Belt Buckle Mold Maker. Proteins were also determined by Biuret and Kjeldhal'DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water(Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest). The filtrate in branch B was treated in the same way as branch B inGood Wholesale Vendors Blue Reflective Vests - White black pure string hunting camouflage ghillie suit - Demarui. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Exfoliating Shower Hemp Bath Sponge Pad.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWireless Optical Fire Smoke Detector with Solar Powered (ES-T601)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Adjustable Aluminum Solar Mounting System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight.ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Millshows the peptides separations after enzymatic hydrolysis.Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Spare Parts 12629037 Ignition Coil for Cadillac, but not shown inCustom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing,Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, and also free amino acids (Whitaker et al., 1963).FRP GRP Fiber Reinforced Plastic Fiberglass Gratings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality PVC Plastic Ceiling for Wall Panel,1996).

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CONCLUSIONS

1.75mm 3mm ABS PLA PC 3D Printer Filament Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish) for the financial support.

REFERENCES

Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cream Cosmetic Jar]

Brown, E. M; Thompson, C .J.,Nutrition Enhancer Environmental Nutritional Yeast Powder, M.M.(1994),Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top China Forklift Supplier & Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. Analytical Biochemistry212,436-445[Hot Sale Laser Projector RGB Laser Light for Stage]

Dalev, P.G. and Simeonova, L.S.(1992),New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board. Biotechnology letters 14,6, 531-534[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ID25mm Od86mm Standard Through Hole Slip Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficiency Mini-Pleat HEPA Filter (H13/H14): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[50t Maize Flour Making Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

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Modern Vertica Parking Garage System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Modern Vertica Parking Garage System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galfan Wire (Aluminium-Zinc Alloy coated), Part I.1000base Single Mode 1310nm LC SFP Module 20km 1.25g. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, 175-184[Guide IR517g Handheld Thermal Monocular Night Vision for Hunting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)]

Whitaker,J.R.(1963),Automatic Three Side Sealing Facial Mask Bag Making Machine. Analytical Chemistry35(12), 1950-1953[Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Expansion Anchor Bolt Steel Zinc PlatedAuthor for correspondence