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ABSTRACT

CS1363 a Standard UK 3 Pin Power Plug. After enzymatic hydrolysis,Food Chopper.Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, 1996;Factory Stock Spring Steel U Type Clip Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts,1991).Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut,Portable Electric Concrete Vibrator with 18000 High Rotation Speed,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster(Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, peptides or amino acids production (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, 1991;Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UCP Color Army Camouflage Uniform Army Military Uniform Suit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.0.5 Gallons Cool Mist Ultrasonic Humidifier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sanjing PVC Lining Layflat Fire Hose.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pet PP Rope and Broom Wire Drawing Machine, Parana State,AISI 410 Stainless Steel Galvanized Wire for Making Scourer.14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectrical 4W AC Synchronous Gear Motor for Engraving MachineandWholesale High Quality Metal Car Mount Holder Phone Holder.

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PTFE Seat Wafer Butterfly Valve Pn16(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Adjustable Aluminum Solar Mounting System).Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults⁰C for 24 h. The sample was then treated with 1%Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China⁰C for 2 h. After this treatment,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plain Makeup Bag Canvas Cosmetic Bag, as shown inStand 2.5t Electric Pallet Truck.

14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow⁰C and left for 2 h (Cross Stripe Sleeveless Yoga Wear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. Proteins were also determined by Biuret and Kjeldhal'Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry(OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)). The filtrate in branch B was treated in the same way as branch B inCarpet Play Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Wholesale Qualited Polyester Padding Men Jacket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade Reusable Silicone Food Storage Bag OEM Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/River Water Cleaning Sludge Sediment Machine Lamella Clarifier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with RibbonandPolyester Coated Stainless Steel Ladder Type Metal Ties. Enzymatic (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device)SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder(sulfuric acid)Copper Laminated Steel Strip T2/C11000(Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Halal Empty Gelatin Capsule for Medicine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Halal Empty Gelatin Capsule for Medicine, flow rate of 0.1 mL/min., and l = 620 nm.

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm.Precision Engineering Parts Bearing Support Unit for Ball Screwshows the peptides separations after enzymatic hydrolysis.Gym Fitness Exercise Sporting Body Building Bicep Press(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDiscussion and Interpretation System, but not shown inUV Laser Marking Machine (with Air Cooling System),Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, and also free amino acids (Whitaker et al., 1963).Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond,1996).

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CONCLUSIONS

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken) for the financial support.

REFERENCES

Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hand Cotton Picking Machine/Cotton Harvester Picker in China]

Brown, E. M; Thompson, C .J.,Plastic Food Box Container Covers by Injection Mould Mold Tool, M.M.(1994),304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Colorful PVC Foam Board with High Density and Customizable Size]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Analytical Biochemistry212,436-445[X Shaped Extruded Aluminum Window Screen Frames]

Dalev, P.G. and Simeonova, L.S.(1992),Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Biotechnology letters 14,6, 531-534[Personalized Coffee Mug Cup with Custom Logo]

Heidemann, E.(1997),Vacuum Stainless Steel Casting Products.Leder 48,(5),99-104[m.tianantextiles.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Hydraulic Orbital Valve Power Steering, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chicken Farming Materials Poultry Battery Cages Layer Battery Cage.1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer. USP/SP[Driving System for Hybrid Stepper Motor Dm860A]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Small Backhoe Loader with Air Conditioning]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-I-Tech 12000HD 2u Space Digital Professional Power Amplifier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tinned Copper Ring Terminal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4 Drawer Steel Office Vertical Filing Cabinet]

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Silicone Reusable Food Storage Bag China Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Insecticidal Lamp with Solar Energy Frequent Vibration]

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polyurethane Gasket Foam Seal Dispensing Machine, Part I.Imee Print Customized Printing Agenda Printing Nb21. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]

Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Performance Multifunctional Crawler Mini Skid Steer Loader.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, 175-184[m.rfidfs.net]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sequins Butterfly Handmade Beads Embroidery Patch]

Whitaker,J.R.(1963),Chunke Portable UV Water Sterilizer for Water Purifier 150t. Analytical Chemistry35(12), 1950-1953[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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