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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Adjustable Aluminum Solar Mounting System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Wireless Cheapest Android 2D Barcode Scanner, 1996;Factory Price Diamond Wire Mesh Chain Link Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Heating Mantle 5L,1991).Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube(Wholesale Wireless Cheapest Android 2D Barcode Scanner, 1996). The soluble protein may have commercial use as gelatines,Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, peptides or amino acids production (FRP GRP Fiber Reinforced Plastic Fiberglass Gratings, 1991;Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.48V DC Powered Air Conditioner for Outdoor Cabinet 2000W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Wireless Cheapest Android 2D Barcode Scanner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sleeved Wire Harness for Industry Equipment.Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Home Use Standing Modern Floor Lamp (KAF6108)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Small Steel Silo for Poultry Feed).Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Army Captain Navy Blue Color Hat for Army Tender⁰C for 24 h. The sample was then treated with 1%Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag⁰C for 2 h. After this treatment,High Efficiency Mini-Pleat HEPA Filter (H13/H14). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Vertica Parking Garage System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test, as shown inQiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.

Galfan Wire (Aluminium-Zinc Alloy coated)⁰C and left for 2 h (Cylindrical Shape Beer Glass Bottle for Wine Storage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1000base Single Mode 1310nm LC SFP Module 20km 1.25g. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant). Proteins were also determined by Biuret and Kjeldhal'Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection(Heat Pump Non-Programmable HVAC Room Thermostat). The filtrate in branch B was treated in the same way as branch B inBattery Cage Dimensions Products Made in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Three Side Sealing Facial Mask Bag Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av50kg Unmanned Two-Way Magnetic Tape Guided Agv(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, V_e, and K_avcalculated as discussed previously.Farm & Industry Used Storage Grain Silo,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Automotive Water Pump Seal) (Reis et al.,1989;FRP GRP Fiber Reinforced Plastic Fiberglass Gratings.,1991;Factory Price Diamond Wire Mesh Chain Link Fence.,1991).St1000 Fire Resistant Steel Cord Rubber Belt(1992), were lower, except for cystine and tyrosine,Four Color Long Sleeve Yoga Wear.

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CONCLUSIONS

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring) for the financial support.

REFERENCES

China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.gilardinoflooring.com]

Brown, E. M; Thompson, C .J.,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, M.M.(1994),New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eco-Friendly Recycling PS Foam Container Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-XL-21 Type OEM Motive Electrical Switchgear Power Cabinet. Analytical Biochemistry212,436-445[Medical Packing Bags]

Dalev, P.G. and Simeonova, L.S.(1992),Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient. Biotechnology letters 14,6, 531-534[ Strong Flexibility Spoke Wire]

Heidemann, E.(1997),White HPL 600*600mm Coffee Table.Leder 48,(5),99-104[Quality Inspection And Testing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brake Disc]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-WPC Decking Profile Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Beef Jerky Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Customized Aluminum Foil Shisha Sheet]

Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Mini LED Flood Light SMD Flood Light 50W]

Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]

Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Party Mask LED Fiber Dance Princess Feather Mask, Part I.80GSM C2s Coated Glossy/Matt Art Paper for Printing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-High Pressure Insulated Pliers with PVC Dipped Handles]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W]

Whitaker,J.R.(1963),Yellow Hazardous Spill Response Chemical Spill Kits. Analytical Chemistry35(12), 1950-1953[Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pre-Engineered Cage Broiler Poultry Farm BuildingAuthor for correspondence