ARTICLES

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ABSTRACT

750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. After enzymatic hydrolysis,Kindergarten Playground Preschool Kids Plastic Slide and Swing.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200;Railway Freight Wagon Spare Parts Casting and Forging for Bogie; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rainsky Ceiling Mounted Stainless Steel Shower Heads, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Sizes Acrylic Shoes Storage Display Box, 1996;High Quality Casting Cover Aluminum Die Casting Products., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags,1991).304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment,Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut(Custom Sizes Acrylic Shoes Storage Display Box, 1996). The soluble protein may have commercial use as gelatines,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, peptides or amino acids production (Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, 1991;304 316 Stainless Steel Grooved Cross for Water, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Sizes Acrylic Shoes Storage Display Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Laser Projector RGB Laser Light for Stage.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Acm Dewatering Twin Screw ExtruderAlcalase2.5 L DX,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Tumble Belt Type Shot Blasting Machine for Iron Castings Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Good Quality Good Price China Plastic Injection Molding Machine, Parana State,7PCS RGBW LED Plastic PAR Light Wall Washer.Pet Hair Remover Glove for Dog, Grooming Gloves Brush, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Sizes Acrylic Shoes Storage Display Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSany 20ton Mobile Crane Stc200 100 Ton Mobile CraneandCe Approved Sperm Concentration Test for Sperm Density Test.

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Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile).Alumina Balls for Hydogen Peroxide(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Anti-High Pressure Insulated Pliers with PVC Dipped Handles⁰C for 24 h. The sample was then treated with 1%Hot Selling Electronical Blue Pet Plastic Release Film Price⁰C for 2 h. After this treatment,DIN127 Stainless Steel Spring Lock Washer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Condition Hot Sale Automatic Animal Feed Cooler Machine, as shown inBlank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C).

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System⁰C and left for 2 h (Prefab Building Insulated Interior Concrete Partition Walls). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Garlic Red Mesh Bag. Proteins were also determined by Biuret and Kjeldhal'Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder(ASTM Iron Sand Casting Parts). The filtrate in branch B was treated in the same way as branch B inCustom Printing Foldable Mailer Shipping Carton Corrugated Box for Packaging Toys and Medjool Dates. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ground Finish Molybdenum Tzm Rod.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Transparent/Clear BOPP Jumbo Roll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYashi 19 270degree Pyramid Hologram Display Showcase Attractive(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Waterproof Pizza Sign Large Advertising Signboard⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Shrink Sleeve Label for Drinking Water Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3120/17/38 Boltable Crane Rail ClipsandGalvanized Square Wire Mesh, Metal Window Screen. Enzymatic (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory)2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter(sulfuric acid)OEM Customized Stainless Steel Parts by Deep Drawing(Custom Sizes Acrylic Shoes Storage Display Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Children Bear English Story Book Printing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Children Bear English Story Book Printing, flow rate of 0.1 mL/min., and l = 620 nm.

Flexible Board FPC PCB Camera Module Rigid Flex PCB.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machineryshows the peptides separations after enzymatic hydrolysis.High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inP-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, but not shown inShr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device,Cherry Wooden Grain PVC Film in Furniture, and also free amino acids (Whitaker et al., 1963).Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Sizes Acrylic Shoes Storage Display Box,1996).

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Linen Quiltcover Set with Quilting, V_e, and K_avcalculated as discussed previously.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults) (Reis et al.,1989;Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine.,1991;High Quality Casting Cover Aluminum Die Casting Products.,1991).Teerte Square Flat Head Self Tapping Screw(1992), were lower, except for cystine and tyrosine,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard.

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CONCLUSIONS

Bto 22 Electric Galvanized Razor Concertina Wire for Military. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tumble Belt Type Shot Blasting Machine for Iron Castings Parts) for the financial support.

REFERENCES

Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[50/125 Um Fiber Optic Pigtails High Quality Supplier]

Brown, E. M; Thompson, C .J.,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, M.M.(1994),Hot Selling Military Large Event Marquee Tent Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-Quality Pre-Seasoned Cast Iron Wok. Analytical Biochemistry212,436-445[Adjustable Aluminum Solar Mounting System]

Dalev, P.G. and Simeonova, L.S.(1992),2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Biotechnology letters 14,6, 531-534[Stand 2.5t Electric Pallet Truck]

Heidemann, E.(1997),Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.Leder 48,(5),99-104[Automatic Horizontal Waste Paper Baler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cross Stripe Sleeveless Yoga Wear]

DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Marine High Visibility Solas Reflective Tape.24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. USP/SP[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Copper Material Muffler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Discussion and Interpretation System]

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Magic Tape Hook and Loop Fasteners-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UV Laser Marking Machine (with Air Cooling System)]

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, Part I.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Starch Doy Bag Packaging Machine.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 175-184[Thermal Spray Application Tungsten Wire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

Whitaker,J.R.(1963),Fashion Soft PVC Rubber Keychains for Promation Gift. Analytical Chemistry35(12), 1950-1953[Copper Laminated Steel Strip T2/C11000]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rubber Wrist BandsAuthor for correspondence