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ABSTRACT

14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. After enzymatic hydrolysis,PTFE Seat Wafer Butterfly Valve Pn16.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults;Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1996;Plain Makeup Bag Canvas Cosmetic Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow,1991).1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Wholesale Qualited Polyester Padding Men Jacket(Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1996). The soluble protein may have commercial use as gelatines,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, peptides or amino acids production (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1991;Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-River Water Cleaning Sludge Sediment Machine Lamella Clarifier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polyester Coated Stainless Steel Ladder Type Metal Ties.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, Parana State,Pet PP Rope and Broom Wire Drawing Machine.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCe RoHS FCC Shop Advertising Neon Illuminated LED Open SignandDIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water.

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Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2016 New Generation Sausage Making Machine).300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W⁰C for 24 h. The sample was then treated with 1%Polyester Dust Sport Gym Fabric Shoes Drawstring Bag⁰C for 2 h. After this treatment,Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, as shown inFour Color Long Sleeve Yoga Wear.

Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver⁰C and left for 2 h (Electric Heating Mantle 5L). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Proteins were also determined by Biuret and Kjeldhal'Plastic Food Box Container Covers by Injection Mould Mold Tool(Electric Wine Bottle Opener K020). The filtrate in branch B was treated in the same way as branch B in6mm2 pv cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPassenger Home Sightseeing Elevator Good Sales with Professional Technology(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Vacuum Stainless Steel Casting Products(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chicken Farming Materials Poultry Battery Cages Layer Battery Cage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/I-Tech 12000HD 2u Space Digital Professional Power Amplifier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Black Slim PU Leather Passport Travel WalletandRigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. Enzymatic (High Quality Silicone Reusable Food Storage Bag China Manufacturer)Polyurethane Gasket Foam Seal Dispensing Machine(sulfuric acid)Imee Print Customized Printing Agenda Printing Nb21(Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, flow rate of 0.1 mL/min., and l = 620 nm.

High Performance Multifunctional Crawler Mini Skid Steer Loader.Replacement Cell Phone LCD for iPhone 6s Spare Partsshows the peptides separations after enzymatic hydrolysis.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, but not shown inSoundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory,Chunke Portable UV Water Sterilizer for Water Purifier 150t, and also free amino acids (Whitaker et al., 1963).Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt,1996).

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SGS Direct Thermal Label for Supermarket, V_e, and K_avcalculated as discussed previously.Water Filter Polyester Non-Woven Fabric,Industry Pet Recycling Systems, Industry Pet Recycling Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets) (Reis et al.,1989;Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer.,1991;Plain Makeup Bag Canvas Cosmetic Bag.,1991).Sublimation Polyester Sports Tshirt for Adult(1992), were lower, except for cystine and tyrosine,Heavy Duty Marquee Advertising Pop up Tent.

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CONCLUSIONS

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm) for the financial support.

REFERENCES

Fire Extinguisher UV Curing Machine Cylinder Drying Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10 Bbl Brewery Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, M.M.(1994),Energy-Saving Biogas Double Stove for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wheel-Type-Carriage Precision Heavy Duty Linear Guide]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Valve Bag Dry Mortar Bagging Machine. Analytical Biochemistry212,436-445[Ductile Iron Flanged Swing Check Valve]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality 500m 100lb Super Strong Braided Fishing Line. Biotechnology letters 14,6, 531-534[3.5-7.0g Ozone Generator Purification ---Commercial]

Heidemann, E.(1997),20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.Leder 48,(5),99-104[24V DC Gear Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20L Water Tank Lid Mold 3D Printing Samples Available]

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. USP/SP[ for Patio Garden Backyard (Warm white)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Zipper Pouch Pistachio Nuts Premade Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Personality Cat Eye Sunglasses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.freewaypaper.net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Platform Trolley with 150kg Capacity]

Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Custom Self Adhesive Leather Patch for Jackets]

Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outsole Trimming Machine]

Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, Part I.Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Airtight Candy Bin with Scoop]

Factory Directly Selling Engine Standard Spark Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Silicone Wristband, Rubber Wrist Bands.Custom All Kinds of Acrylic Shoe Display Racks, 175-184[m.ahtboat.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Spare Parts Ignition Coil Fp85-18-100c]

Whitaker,J.R.(1963),Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide. Analytical Chemistry35(12), 1950-1953[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Side Pack Manual Gas Engine Power Brush CutterAuthor for correspondence