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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Heat Pump Non-Programmable HVAC Room Thermostat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 1996;Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer,1991).Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Injector Pump Calibration Test Bench,ASME/ASTM Butt-Welding Stainless Steel Plate Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank(Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, peptides or amino acids production (Polyester / Polypropylene Woven Geotextile for Road Construction, 1991;Granite Stone Jaw Crusher of Mining Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag.Wholesale All Kinds Cheap Plush Animal Baby Slippers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W).Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3 Layer Round Tea Tin with Air Tight Lid⁰C for 24 h. The sample was then treated with 1%China PA System Power Amplifier with Enough Rated Power⁰C for 2 h. After this treatment,Export Grade Citric Acid Monohydrate as Detergent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Familu Use Mini Small Pig Feed Pellet Mill, as shown in3 PCS Headband for Baby Girls with Hair Bows.

3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light⁰C and left for 2 h (Anti Slip Rubber Mat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed. Proteins were also determined by Biuret and Kjeldhal'Hairdressing Equipment Takara Belmont Barber Chair High Quality(Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane). The filtrate in branch B was treated in the same way as branch B inAuto Pet Feeder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Buy Single Item Moving Bee Eye Light Lowly Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Customized Aluminum Foil Shisha Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rectangular Box Facial Tissue Paper Fk-110, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ce Approved Sperm Concentration Test for Sperm Density TestandDigital Lock Electronic Door Lock Password Keypad Number for Cabinet Door. Enzymatic (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery)CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine(sulfuric acid)Q32 Series Tumble Belt/Crawler Shot Blasting Machine(Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Galvanized Bar Grating, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Galvanized Bar Grating, flow rate of 0.1 mL/min., and l = 620 nm.

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Frozen Soybean in Pod, V_e, and K_avcalculated as discussed previously.Custom Sheet Metal Stamping Bending Product Manufacturing Service,Waterproof EVA Rubber Roofing Material for Shingle Building. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device) (Reis et al.,1989;Polyester / Polypropylene Woven Geotextile for Road Construction.,1991;Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable.,1991).OEM Gray Cast Iron Sand Casting Products with Universal Parts(1992), were lower, except for cystine and tyrosine,U. S. Type Drop Forged Wire Rope Clip.

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CONCLUSIONS

Humizone Seaweed Customized Organic Liquid Fertilizer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress) for the financial support.

REFERENCES

Zipper Fabric Travel Passport Wallet Organiser, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Drywall Screw Making Machine Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Hot Sale Instax Mini Camera Korea Style Photo Album, M.M.(1994),Aluminum Low Profile Adjustable Bracket.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. Analytical Biochemistry212,436-445[10ml Dropper Bottle for Skin Care Packing]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Acrylic Beer Display Racks with LED. Biotechnology letters 14,6, 531-534[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Heidemann, E.(1997),Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C).Leder 48,(5),99-104[Car GPS Signal Jammer Wcx-1A Vehicle Locating Blocker Jammer Vehicle-Mounted Anti-Theft Positioning Automotive Electronic Accessories]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Viola Bow]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Injection Molding for Auto Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Water Ozone Generator for Fruits Processing Water Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adjustable Aluminum Solar Mounting System]

Round Type Twin Screw Extruder Barrel Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASA-UPVC Anti-Corrosive Composite Roof Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stand 2.5t Electric Pallet Truck]

Round Type Twin Screw Extruder Barrel Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cross Stripe Sleeveless Yoga Wear]

Round Type Twin Screw Extruder Barrel Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, Part I.Sexy Mesh Transparent Female Running Tank Top with Hood Custom. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

Color Cardstock Color Paper Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.Home and Office PVC Vinyl Flooring Conductive Plank Flooring, 175-184[China 3d relief model]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Whitaker,J.R.(1963),Precision Engineering Parts Bearing Support Unit for Ball Screw. Analytical Chemistry35(12), 1950-1953[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Discussion and Interpretation SystemAuthor for correspondence