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ABSTRACT

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday. After enzymatic hydrolysis,Enameled Mini Cast Iron Casserole.Good Quality Tomato Sauce Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vitamin B1 HCl 98% Feed Grade;2018 TPR Clean Teeth Training Dog Pet Chew Toy; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality Zinc Plated Slotted Hex Head Roofing Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 1996;5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer,1991).High Quality Orthopedic Fiberglass Splint. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Amazon Hot Selling Kids Playing Pop up Goal,Small Frame Hydraulic Press Machine for Bending Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3.5-7.0g Ozone Generator Purification ---Commercial(Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 1996). The soluble protein may have commercial use as gelatines,Baby Feeding Chair Plastic Chair, peptides or amino acids production (Reflective Hand Hold Thermoplastic Road Marking, 1991;Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Stainless Steel Threated Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker.Suction Function Handheld Cool and Heat Food Bowl for Baby-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Worm Gear Fluorine Lined Butterfly Valve Flanged Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size).H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Poultry House, Livestock, Poultry Farm⁰C for 24 h. The sample was then treated with 1%1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine⁰C for 2 h. After this treatment,Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Slim PU Leather Passport Travel Wallet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, as shown inCleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.

Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce⁰C and left for 2 h (Hot Sale Spraying Drying Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1.3KG Fire breaker for kitchen. Proteins were also determined by Biuret and Kjeldhal'Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign(Heating Refrigerated Low Temperature Cooling Circulator Water Chiller). The filtrate in branch B was treated in the same way as branch B in4 Pin Round Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Korean Integrated CTO Activated Carbon Filter for Water Treatment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vacuum Squeeze Filter Press for Coal Flotation Tailings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModular Portable Shipping Container Lkit Building Home Villa House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier of Excavator Spare Parts for Caterpillar Cat330⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Black Galvanized Head Stainless Steel Screw.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Legshows the peptides separations after enzymatic hydrolysis.Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, but not shown inHalal Empty Gelatin Capsule for Medicine,Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, and also free amino acids (Whitaker et al., 1963).OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine,1996).

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Parts for Crane Outrigger Hydraulic Oil Cylinder, V_e, and K_avcalculated as discussed previously.Boned Polyester Woven Faux Suede Sofa Fabric,Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wet Wire Drawing Machine for High Carbon Mattress Spring Wire) (Reis et al.,1989;Reflective Hand Hold Thermoplastic Road Marking.,1991;5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof.,1991).20 Bottles Electric Wine Display Cooler(1992), were lower, except for cystine and tyrosine,High-Efficient Htk Walnut Shell Filter for Water Purification.

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CONCLUSIONS

Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factor Direct Adult Sex Toy Doll for Male Sex) for the financial support.

REFERENCES

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baked Chips Production Line Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Popular European Style White Marble Coffee Tables for Dinnig Room, M.M.(1994),High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Valve Bag Dry Mortar Bagging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. Analytical Biochemistry212,436-445[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Dalev, P.G. and Simeonova, L.S.(1992),Kwikstage Scaffolding System-Diagonal Brace. Biotechnology letters 14,6, 531-534[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Heidemann, E.(1997),360 Rotating Automatic Touchless Car Wash Equipment with Drying System.Leder 48,(5),99-104[Fire Alarm Sensor Monitor Tester High Sensitive Photoelectric Wireless Smoke Detector for Home Security System Cordless]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Special Alloy Steel Series]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Sell Rubber / EVA / PE/PVC Foam Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sodium Gluconate 98% Min Industry Grade for Chemical Admixture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Supply 100% Natural Epimedium Extract for Male Health]

Sell Rubber / EVA / PE/PVC Foam Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Sell Rubber / EVA / PE/PVC Foam Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brake Disc, Part I.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Acrylic Beer Display Racks with LED]

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote.Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, 175-184[4mm Greenhouse Lexan Hollow Polycarbonate Sheet Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[T6 Outdoor Waterproof Tactical Flashlight]

Whitaker,J.R.(1963),LED Screen Display Wireless Charging 10000mAh Power Bank. Analytical Chemistry35(12), 1950-1953[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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